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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 15, Iss. 11 — May. 28, 2007
  • pp: 7066–7074

Novel fluorescent probe for low density lipoprotein, based on the enhancement of Europium emission band

L. C. Courrol, A.M. Monteiro, F.R.O. Silva, L. Gomes, N.D. Vieira, Jr., M.A. Gidlund, and A.M. Figueiredo Neto  »View Author Affiliations

Optics Express, Vol. 15, Issue 11, pp. 7066-7074 (2007)

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We report here the observation of the enhancement of Europium-tetracycline complex emission in Low Density Lipoprotein (LDL) solutions. Europium emission band of tetracycline solution containing Europium (III) chloride hexahydrate was tested to obtain effective enhancement in the presence of native LDL and oxidized LDL. Europium emission lifetime in the presence of lipoproteins was measured, resulting in a simple method to measure the lipoproteins quantity in an aqueous solution at physiological pH. This method shows that the complex can be used as a sensor to determine the different states of native and oxidized LDL in biological fluids.

© 2007 Optical Society of America

OCIS Codes
(170.1470) Medical optics and biotechnology : Blood or tissue constituent monitoring
(170.3650) Medical optics and biotechnology : Lifetime-based sensing
(300.6280) Spectroscopy : Spectroscopy, fluorescence and luminescence

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: April 5, 2007
Revised Manuscript: May 15, 2007
Manuscript Accepted: May 16, 2007
Published: May 24, 2007

Virtual Issues
Vol. 2, Iss. 6 Virtual Journal for Biomedical Optics

L. C. Courrol, A. M. Monteiro, F. R. O. Silva, L. Gomes, N. D. Vieira Jr., M. A. Gidlund, and A. M. Figueiredo Neto, "Novel fluorescent probe for low density lipoprotein, based on the enhancement of Europium emission band," Opt. Express 15, 7066-7074 (2007)

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