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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 15, Iss. 12 — Jun. 11, 2007
  • pp: 7231–7242

Real-time dual-wavelength digital holographic microscopy with a single hologram acquisition

Jonas Kühn, Tristan Colomb, Frédéric Montfort, Florian Charrière, Yves Emery, Etienne Cuche, Pierre Marquet, and Christian Depeursinge  »View Author Affiliations

Optics Express, Vol. 15, Issue 12, pp. 7231-7242 (2007)

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A technique to perform two-wavelengths digital holographic microscopy (DHM) measurements with a single hologram acquisition is presented. The vertical measurement range without phase ambiguity is extended to the micron-range, thanks to the resulting synthetic wavelength defined by the beating of two wavelengths with a separation of about 80nm. Real-time dual-wavelength imaging is made possible by using two reference waves having different wavelengths and propagation directions for the hologram recording. The principle of the method is exposed and experimental results concerning a 1.2μm m high test sample as well as a moving micro-mirror are presented. To the extent of our knowledge, this is the first time that real-time synthetic beat-wavelength digital holography measurements are reported.

© 2007 Optical Society of America

OCIS Codes
(090.1760) Holography : Computer holography
(090.4220) Holography : Multiplex holography
(120.5050) Instrumentation, measurement, and metrology : Phase measurement
(180.3170) Microscopy : Interference microscopy

ToC Category:

Original Manuscript: April 6, 2007
Revised Manuscript: May 16, 2007
Manuscript Accepted: May 22, 2007
Published: May 29, 2007

Virtual Issues
Vol. 2, Iss. 7 Virtual Journal for Biomedical Optics

Jonas Kühn, Tristan Colomb, Frédéric Montfort, Florian Charrière, Yves Emery, Etienne Cuche, Pierre Marquet, and Christian Depeursinge, "Real-time dual-wavelength digital holographic microscopy with a single hologram acquisition," Opt. Express 15, 7231-7242 (2007)

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  1. J. W. Goodman and R. W. Lawrence, "Digital image formation from electronically detected holograms," Appl. Phys. Lett. 11, 77-79 (1967). [CrossRef]
  2. U. Schnars and W. Jüptner, "Direct recording of holograms by a ccd target and numerical reconstruction," Appl. Opt. 33, 179-181 (1994). [CrossRef] [PubMed]
  3. E. Cuche, F. Bevilacqua, and C. Depeursinge, "Digital holography for quantitative phase-contrast imaging," Opt. Lett. 24, 291-293 (1999). [CrossRef]
  4. D. Gabor, "A new microscopic principle," Nature 161, 777-778 (1948). [CrossRef] [PubMed]
  5. E. Cuche, P. Marquet, and C. Depeursinge, "Simultaneous amplitude-contrast and quantitative phase-contrast microscopy by numerical reconstruction of fresnel off-axis holograms," Appl. Opt. 38, 6994-7001 (1999). [CrossRef]
  6. A. T. Forrester, W. E. Parkins, and E. Gerjuoy, "On the possibility of observing beat frequencies between lines in the visible spectrum," Phys. Rev. 72, 728-728 (1947). [CrossRef]
  7. C. Polhemus, "Two-wavelength interferometry," Appl. Opt. 12, 2071-2074 (1973). [CrossRef] [PubMed]
  8. F. Bien, M. Camac, H. J. Caulfield, and S. Ezekiel, "Absolute distance measurements by variable wavelength interferometry," Appl. Opt. 20, 400-402 (1981). [CrossRef] [PubMed]
  9. R. Dändliker, R. Thalmann, and D. Prongué, "Two-wavelength laser interferometry using superheterodyne detection," Opt. Lett. 13, 339-341 (1988). [CrossRef] [PubMed]
  10. B. P. Hildebrand and K. A. Haines, "Multiple-wavelength and multiple-source holography applied to contour generation," J. Opt. Soc. Am. 57, 155-162 (1967). [CrossRef]
  11. J. S. Zelenka and J. R. Varner, "A new method for generating depth contours holographically," Appl. Opt. 7, 2107-2110 (1968). [CrossRef] [PubMed]
  12. J. C. Wyant, "Testing aspherics using two-wavelength holography," Appl. Opt. 10, 2113-2118 (1971). [CrossRef] [PubMed]
  13. C. Wagner, W. Osten, and S. Seebacher, "Direct shape measurement by digital wavefront reconstruction and multiwavelength contouring," Opt. Eng. 39, 79-85 (2000). [CrossRef]
  14. J. Gass, A. Dakoff, and M. K. Kim, "Phase imaging without 2 pi ambiguity by multiwavelength digital holography," Opt. Lett. 28, 1141-1143 (2003). [CrossRef] [PubMed]
  15. D. Parshall and M. Kim, "Digital holographic microscopy with dual wavelength phase unwrapping," Appl. Opt. 45, 451-459 (2006). [CrossRef] [PubMed]
  16. T. Colomb, F. Montfort, J. Kühn, N. Aspert, E. Cuche, A. Marian, F. Charrière, S. Bourquin, P. Marquet, and C. Depeursinge, "Numerical parametric lens for shifting, magnification and complete aberration compensation in digital holographic microscopy," J. Opt. Soc. Am. A 23, 3177-3190 (2006). [CrossRef]
  17. S. de Nicola, A. Finizio, G. Pierattini, D. Alfieri, S. Grilli, L. Sansone, and P. Ferraro, "Recovering correct phase information in multiwavelength digital holographic microscopy by compensation for chromatic aberrations," Opt. Lett. 30, 2706-2708 (2005). [CrossRef] [PubMed]
  18. I. Yamaguchi, K. Yamamoto, G. A. Mills, and M. Yokota, "Image reconstruction only by phase data in phaseshifting digital holography," Appl. Opt. 45, 975-983 (2006). [CrossRef] [PubMed]
  19. R. J. Mahon, J. A. Murphy, and W. Lanigan, "Digital holography at millimetre wavelengths," Opt. Commun. 260, 469-473 (2006). [CrossRef]
  20. F. Zhang, G. Pedrini, and W. Osten, "Reconstruction algorithm for high-numerical-aperture holograms with diffraction-limited resolution," Opt. Lett. 31, 1633-1635 (2006). [CrossRef] [PubMed]
  21. R. Onodera and Y. Ishii, "Two-wavelength interferometry that uses a fourier-transform method," Appl. Opt. 37, 7988-7994 (1998). [CrossRef]
  22. A. W. Lohmann, "Reconstruction of vectorial wavefronts," Appl. Opt. 4, 1667-1668 (1965). [CrossRef]
  23. E. N. Leith and J. Upatnieks, "Wavefront reconstruction with diffused illumination and three-dimensional objects," J. Opt. Soc. Am. 54, 1295-1301 (1964). [CrossRef]
  24. E. N. Leith and J. Upatnieks, "Wavefront reconstruction 1377-1381 (1963). [CrossRef]
  25. J. D. Armitage and A. W. Lohmann, "Theta modulation in optics," Appl. Opt. 4, 400-403 (1965). [CrossRef]
  26. D. Beghuin, E. Cuche, P. Dahlgren, C. Depeursinge, G. Delacretaz, and R. P. Salathe, "Single acquisition polarization imaging with digital holography," Electron. Lett. 35, 2053-2055 (1999). [CrossRef]
  27. T. Colomb, P. Dahlgren, D. Beghuin, E. Cuche, P. Marquet, and C. Depeursinge, "Polarization imaging by use of digital holography," Appl. Opt. 41, 27-37 (2002). [CrossRef] [PubMed]
  28. T. Colomb, E. Cuche, F. Montfort, P. Marquet, and C. Depeursinge, "Jones vector imaging by use of digital holography: simulation and experimentation," Opt. Commun. 231, 137-147 (2004). [CrossRef]
  29. A. T. Saucedo, F. M. Santoyo, M. D. l. Torre-Ibarra, G. Pedrini, and W. Osten, "Endoscopic pulsed digital holography for 3d measurements," Opt. Lett. 14, 1468-1475 (2006).
  30. T. Saucedo A., F. M. Santoyo, M. D. l. T. Ibarra, G. Pedrini, and W. Osten, "Simultaneous two-dimensional endoscopic pulsed digital holography for evaluation of dynamic displacements," Appl. Opt. 45, 4534-4539 (2006). [CrossRef] [PubMed]
  31. E. Cuche, P. Marquet, and C. Depeursinge, "Spatial filtering for zero-order and twin-image elimination in digital off-axis holography," Appl. Opt. 39, 4070-4075 (2000). [CrossRef]
  32. T. Colomb, E. Cuche, F. Charrière, J. K¨uhn, N. Aspert, F. Montfort, P. Marquet, and C. Depeursinge, "Automatic procedure for aberration compensation in digital holographic microscopy and applications to specimen shape compensation," Appl. Opt. 45, 851-863 (2006). [CrossRef] [PubMed]
  33. P. de Groot and S. Kishner, "Synthetic wavelength stabilization for two-color laser-diode interferometry," Appl. Opt. 30, 4026-4033 (1991). [CrossRef] [PubMed]
  34. Y. Emery, E. Cuche, F. Marquet, N. Aspert, P. Marquet, J. Kühn, M. Botkine, T. Colomb, F. Montfort, F. Charrière, C. Depeursinge, P. Debergh, and R. Conde, "Digital holographic microscopy (DHM) for metrology and dynamic characterization of MEMS and MOEMS," Proc. SPIE 6186, N1860-N1860 (2006).

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