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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 15, Iss. 13 — Jun. 25, 2007
  • pp: 8115–8124

Investigating nanoscale cellular dynamics with cross-sectional spectral domain phase microscopy

Audrey K. Ellerbee, Tony L. Creazzo, and Joseph A. Izatt  »View Author Affiliations


Optics Express, Vol. 15, Issue 13, pp. 8115-8124 (2007)
http://dx.doi.org/10.1364/OE.15.008115


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Abstract

We report on cross-sectional imaging of dynamic biological specimens using a spectral domain phase microscopy (SDPM) system capable of operating at a line rate of 19 kHz. This system combines the time-sensitive capabilities of SDPM with the multi-point acquisition features of related phase-sensitive techniques. The presented phase portraits and B-scan phase images of spontaneously beating embryonic cardiomyocytes and cytoplasmic flow in A. proteus offer insight into the nature and timing of the observed cellular phenomena, demonstrating the utility of this technique for dynamic cell studies.

© 2007 Optical Society of America

OCIS Codes
(100.5070) Image processing : Phase retrieval
(110.0180) Imaging systems : Microscopy
(110.4500) Imaging systems : Optical coherence tomography
(120.5050) Instrumentation, measurement, and metrology : Phase measurement

ToC Category:
Microscopy

History
Original Manuscript: April 19, 2007
Revised Manuscript: June 4, 2007
Manuscript Accepted: June 8, 2007
Published: June 13, 2007

Virtual Issues
Vol. 2, Iss. 7 Virtual Journal for Biomedical Optics

Citation
Audrey K. Ellerbee, Tony L. Creazzo, and Joseph A. Izatt, "Investigating nanoscale cellular dynamics with cross-sectional spectral domain phase microscopy," Opt. Express 15, 8115-8124 (2007)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-15-13-8115


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References

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