OSA's Digital Library

Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 15, Iss. 15 — Jul. 23, 2007
  • pp: 9239–9247

LED-based multi-wavelength phase imaging interference microscopy

N. Warnasooriya and M. K. Kim  »View Author Affiliations


Optics Express, Vol. 15, Issue 15, pp. 9239-9247 (2007)
http://dx.doi.org/10.1364/OE.15.009239


View Full Text Article

Enhanced HTML    Acrobat PDF (3837 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

LED-based multi-wavelength phase imaging interference microscopy combines phase-shifting interferometry with multi-wavelength optical phase unwrapping. This technique consists of a Michelson-type interferometer illuminated with a LED. The reference mirror is dithered for obtaining interference images at four phase quadratures, which are then combined to calculate the phase of the object surface. The 2π ambiguities are removed by repeating the experiment using two or more LEDs at different wavelengths, which yields phase images of effective wavelength much longer than the original. The resulting image is a profile of the object surface with a height resolution of several nanometers and range of several microns. The interferographic images using broadband sources are significantly less affected by coherent noise.

© 2007 Optical Society of America

OCIS Codes
(120.3180) Instrumentation, measurement, and metrology : Interferometry
(120.5050) Instrumentation, measurement, and metrology : Phase measurement
(180.3170) Microscopy : Interference microscopy

ToC Category:
Microscopy

History
Original Manuscript: May 30, 2007
Revised Manuscript: July 10, 2007
Manuscript Accepted: July 10, 2007
Published: July 12, 2007

Virtual Issues
Vol. 2, Iss. 8 Virtual Journal for Biomedical Optics

Citation
N. Warnasooriya and M. K. Kim, "LED-based multi-wavelength phase imaging interference microscopy," Opt. Express 15, 9239-9247 (2007)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-15-15-9239


Sort:  Author  |  Year  |  Journal  |  Reset  

References

  1. E. D. Barone-Nugent, A. Barty, K. A. Nugent, "Quantitative phase amplitude microscopy I: optical microscopy," J. Microscopy 3, 194-203 (2002). [CrossRef]
  2. C. J. Mann, L. Yu, C. Lo and M. K. Kim, "High-resolution quantitative phase-contrast microscopy by digital holography," Opt. Express 13, 8693-8698 (2005). [CrossRef] [PubMed]
  3. P. Marquet, B. Rappaz, T. Colomb, F. Charriere, J. Kuhn, Y. Emery, E. Cuche, C. Depeursinge, P. Magistretti, " Digital holographic microscopy, a new optical imaging technique to investigate cellular dynamics," Proc. SPIE 6191, 61910U-61910U5 (2006). [CrossRef]
  4. Y. Park, G. Popescu, K. Badizadegan, R. R. Dasari and M. S. Feld, "Diffraction phase and fluorescence microscopy," Opt. Express 14, 8263-8268 (2006). [CrossRef] [PubMed]
  5. M. Servin, J.L. Marroquin, D. Malacara and F.J. Cuevas, "Phase unwrapping with a regularized phase-tracking system," Appl. Opt. 37, 1917-1923 (1998). [CrossRef]
  6. Yeou-Yen Cheng and James C. Wyant, "Multiple-wavelength phase-shifting interferometry," Appl. Opt. 24, 804-807 (1985). [CrossRef] [PubMed]
  7. C. Wagner, W. Osten and S. Seebacher, "Direct shape measurement by digital wavefront reconstruction and multiwavelength contouring," Opt. Eng. 39, 79-85, (2000). [CrossRef]
  8. J. Gass, A. Dakoff, M.K. Kim, "Phase imaging without 2π ambiguity by multiwavelength digital holography," Opt. Lett. 28, 1141-1143, (2003). [CrossRef] [PubMed]
  9. D. Parshall and M. K. Kim, "Digital holographic microscopy with dual wavelength phase unwrapping," Appl. Opt. 45, 451-459 (2006). [CrossRef]
  10. M. Tziraki, R. Jones, P. M. W. French, M. R. Melloch and D. D. Nolte, "Photorefractive holography for imaging through turbid media using low coherent light," Appl. Phy. B 70, 151-154 (2000). [CrossRef]
  11. S. Dilhaire, S. Grauby, S. Jorez, L. D. P. Lopez, J. Rampnoux and W. Claeys, " Surface displacement imaging by interferometry with a light emitting diode," Appl. Opt. 41, 4996-5001 (2002). [CrossRef] [PubMed]
  12. L. Repetto, E. Piano and C. Pontiggia, "Lensless digital holographic microscope with light-emitting diode illumination," Opt. Lett. 29, 1132-1134 (2004). [CrossRef] [PubMed]
  13. A. Fercher, W. Drexler, C. K. Hitzenberger and T. Lasser, "Optical coherence tomography-principles and applications," Rep. Prog. Phys. 66, 239-303 (2003). [CrossRef]
  14. LuxeonTM Emitter and Star sample information AB11, 2 (Feb 2002).

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.


« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited