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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 15, Iss. 16 — Aug. 6, 2007
  • pp: 10123–10134

Differential imaging in coherent anti-Stokes Raman scattering microscopy with Laguerre-Gaussian excitation beams

Cheng Liu and Dug Young Kim  »View Author Affiliations

Optics Express, Vol. 15, Issue 16, pp. 10123-10134 (2007)

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We propose a new differential imaging technique to visualize the fine structures and the edges of a sample in coherent anti-Stokes Raman Scattering (CARS) microscopy. Both the pump and Stokes excitation fields are modulated simultaneously with a spiral phase mask which transforms them from Gaussian modes into Laguerre-Gaussian modes of LG01 for CARS excitation. With an accurate three dimensional finite-difference time-domain (FDTD) method, the intensity and phase distributions of focused input fields, the scattering pattern of generated CARS signal as well as the formation of differential images are studied detailedly, and by simulating the sensitivity range and reliability of this method, we have verified that it is much suitable for visualizing structures with a scale comparable to the excitation wavelength and has higher reliability in retrieving chemical structural information of the sample compared to common CARS microscopy.

© 2007 Optical Society of America

OCIS Codes
(180.1790) Microscopy : Confocal microscopy
(290.5860) Scattering : Scattering, Raman
(300.6230) Spectroscopy : Spectroscopy, coherent anti-Stokes Raman scattering
(330.6130) Vision, color, and visual optics : Spatial resolution

ToC Category:

Original Manuscript: June 21, 2007
Revised Manuscript: July 23, 2007
Manuscript Accepted: July 24, 2007
Published: July 26, 2007

Virtual Issues
Vol. 2, Iss. 9 Virtual Journal for Biomedical Optics

Liu Cheng and Dug Y. Kim, "Differential imaging in coherent anti-Stokes Raman scattering microscopy with Laguerre- Gaussian excitation beams," Opt. Express 15, 10123-10134 (2007)

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