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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 15, Iss. 18 — Sep. 3, 2007
  • pp: 11167–11177

In vivo and ex vivo imaging of intra-tissue elastic fibers using third-harmonic-generation microscopy

Che-Hang Yu, Shih-Peng Tai, Chun-Ta Kung, I-Jong Wang, Han-Chieh Yu, Hsiang-Ju Huang, Wen-Jeng Lee, Yi-Fan Chan, and Chi-Kuang Sun  »View Author Affiliations

Optics Express, Vol. 15, Issue 18, pp. 11167-11177 (2007)

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Elastin is an essential and widespread structural protein in charge of the integrity on tissues and organs. In this study, we demonstrate that elastin is a major origin of the third-harmonic-generation (THG) contrast under Cr:forsterite laser excitation operating at 1230nm, with selective visualization inside many tissues such as lung tissues and arteries. In vivo imaging of the nude mouse elastic cartilage beneath the hypodermis by epi-THG microscopy keeps the high resolution and contrast in all three dimensions. Combined with second-harmonic-generation microscopy, THG microscopy exhibits the ability to show the extraordinary proliferation of elastic fibers for the ophthalmic disease of pterygium and the capability of distinguishable visualization from collagen.

© 2007 Optical Society of America

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(180.6900) Microscopy : Three-dimensional microscopy
(190.4160) Nonlinear optics : Multiharmonic generation
(190.4180) Nonlinear optics : Multiphoton processes

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: June 15, 2007
Revised Manuscript: July 24, 2007
Manuscript Accepted: July 24, 2007
Published: August 21, 2007

Virtual Issues
Vol. 2, Iss. 10 Virtual Journal for Biomedical Optics

Chi-Kuang Sun, Che-Hang Yu, Shih-Peng Tai, Chun-Ta Kung, I-Jong Wang, Han-Chieh Yu, Hsiang-Ju Huang, Wen-Jeng Lee, and Yi-Fan Chan, "In vivo and ex vivo imaging of intra-tissue elastic fibers using third-harmonic-generation microscopy," Opt. Express 15, 11167-11177 (2007)

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