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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 15, Iss. 18 — Sep. 3, 2007
  • pp: 11178–11184

Monitoring thermally-induced phase transitions in porcine cornea with the use of fluorescence micro-imaging analysis

F. Rossi, P. Matteini, I. Bruno, P. Nesi, and R. Pini  »View Author Affiliations

Optics Express, Vol. 15, Issue 18, pp. 11178-11184 (2007)

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Thermal modifications induced in corneal stroma were investigated with the use of fluorescence microscopy. Tissue samples were heated in a water bath at temperatures in the 35–90°C range. Fluorescence images of the structural modifications induced were acquired after staining with Indocyanine Green (ICG). Discrete Fourier Transform (DFT) and entropy analyses of each image made it possible to characterize the thermally-induced phase transitions in the stroma, and to indicate a threshold value for high thermal damage. The procedure could be proposed as the basis for a real-time controlling system for surgical techniques based on induced thermal effects.

© 2007 Optical Society of America

OCIS Codes
(100.2960) Image processing : Image analysis
(120.6810) Instrumentation, measurement, and metrology : Thermal effects
(170.2520) Medical optics and biotechnology : Fluorescence microscopy

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: June 4, 2007
Revised Manuscript: July 25, 2007
Manuscript Accepted: July 25, 2007
Published: August 21, 2007

Virtual Issues
Vol. 2, Iss. 10 Virtual Journal for Biomedical Optics

F. Rossi, P. Matteini, I. Bruno, P. Nesi, and R. Pini, "Monitoring thermally-induced phase transitions in porcine cornea with the use of fluorescence micro-imaging analysis," Opt. Express 15, 11178-11184 (2007)

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  1. R. Brinkmann, N. Koop, G. Geerling, J. Kampmeier, S. Borcherding, K. Kamm, R. Birngruber, "Diode laser thermokeratoplasty: application strategy and dosimetry," J. Cataract Refract. Surg. 24, 1195-207 (1998). [PubMed]
  2. F. Rossi, R. Pini, L. Menabuoni, R. Mencucci, U. Menchini, S. Ambrosini, G. Vannelli, "Experimental study on the healing process following laser welding of the cornea," J. Biomed. Opt. 10, 024004 (2005). [CrossRef] [PubMed]
  3. K. Suthamjariya and R. Rox Anderson, "Laser in Dermatology," in Biomedical Photonics Handbook, T. Vo-Dinh, Ed., (CRC Press 2003), pp. 40/1-40/40.
  4. A. L. Wallace, R. M. Hollinshead, and C. B. Franck, "The scientific basis of thermal capsular shrinkage," J. Shoulder Elbow Surg. 9, 354-360 (2000). [CrossRef] [PubMed]
  5. J. Kampmeier, B. Radt, R. Birngruber, and R. Brinkmann, "Thermal and biomechanical parameters of porcine cornea," Cornea 19, 355-363 (2000). [CrossRef] [PubMed]
  6. C. A. Miles, T. V. Burjanadze, and A. J. Bailey, "The kinetics of the thermal denaturation of collagen in unrestrained rat tail tendon determined by differential scanning calorimetry," J. Mol. Biol. 245, 437-446 (1995). [CrossRef] [PubMed]
  7. F. Flandin, C. Buffevant, and D. Herbage, "A differential scanning calorimetry analysis of the age-related changes in the thermal stability of rat skin collagen," Biochim. Biophys. Acta 791, 205-211 (1984). [CrossRef] [PubMed]
  8. P. Kronick, B. Maleeff, and R. Carroll, "The locations of collagens with different thermal stabilities in fibrils of bovine reticular dermis," Connect. Tissue Res. 18, 123-134 (1988). [CrossRef] [PubMed]
  9. R. Agah, J. A. Pearce, A. J. Welch, and M. Motamedi, "Rate process model for arterial tissue thermal damage: implications on vessel photocoagulation," Lasers Surg. Med. 15, 176-184 (1994). [CrossRef] [PubMed]
  10. A. D. Zweig, B. Meierhofer, O. M. Muller, C. Mischler, V. Romano, M. Frenz, and H. P. Weber, "Lateral thermal damage along pulsed laser incisions," Lasers Surg. Med. 10, 262-274 (1990). [CrossRef] [PubMed]
  11. T. Tanaka, S. Furutani-Miura, M. Nakamura, and T. Nishida, "Immunohistochemical study of localization of extracellular matrix after holmium YAG laser irradiation in rat cornea," Jpn. J. Ophthalmol. 44, 482-488 (2000). [CrossRef] [PubMed]
  12. S. J. Lin, C. Y. Hsiao, Y. Sun, W. Lo, W. C. Lin, G. J. Jan, S. H. Jee, C. Y. Dong, "Monitoring the thermally induced structural transitions of collagen by use of second-harmonic generation microscopy," Opt. Lett. 30, 622-624 (2005). [CrossRef] [PubMed]
  13. T. Theodossiou, G. S. Rapti, V. Hovhannisyan, E. Georgiou, K. Politopoulos, and D. Yova, "Thermally induced irreversible conformational changes in collagen probed by optical second harmonic generation and laser-induced fluorescence," Lasers Med. Sci. 17, 34-41 (2002). [CrossRef] [PubMed]
  14. M. G. Lin, T. L. Yang, C. T. Chiang, H. C. Kao, J. N. Lee, W. Lo, S. H. Jee, Y. F. Chen, C. Y. Dong, and S. J. Lin, "Evaluation of dermal thermal damage by multiphoton autofluorescence and second-harmonic-generation microscopy," J. Biomed. Opt. 11, 064006 (2006). [CrossRef]
  15. G. Cox, E. Kable, A. Jones, I. Fraser, F. Manconi, and M. D. Gorrell, "3-dimensional imaging of collagen using second harmonic generation," J. Struct. Biol. 141, 53-62 (2003). [CrossRef] [PubMed]
  16. H. Y. Tan, S. W. Teng, W. Lo, W. C. Lin, S. J. Lin, S. H. Jee, and C. Y. Dong, "Characterizing the thermally induced structural changes to intact porcine eye, part 1: second harmonic generation imaging of cornea stroma," J. Biomed. Opt. 10, 054019 (2005). [CrossRef] [PubMed]
  17. G. Wollensak, E. Spoerl, and T. Seiler, "Stress-strain measurements of human and porcine corneas after riboflavin-ultraviolet-A-induced cross-linking," J. Cataract Refract. Surg. 29, 1780-1785 (2003) [CrossRef] [PubMed]
  18. M. L. J. Landsman, G. Kwant, G. A. Mook, and W. G. Zijlstra, "Light- absorbing properties, stability, and spectral stabilization of indocyanine green," J. Appl. Physiol. 40, 575-583 (1976). [PubMed]
  19. L.-P. Kamolza, H. Andel, W. Haslik, A. Donner, W. Winter, G. Meissl, and M. Frey, "Indocyanine green video angiographies help to identify burns requiring operation," Burns 29, 785-791 (2003). [CrossRef]
  20. L. A. Yannuzzi, M. D. Ober, J. S. Slakter, R. F. Spaide, Y L. Fisher, R.W. Flower, R. Rosen, "Ophthalmic fundus imaging: today and beyond," Am. J. Ophthalmol. 137, 511-524 (2004). [CrossRef] [PubMed]
  21. G. P. Holley, A. Alam, A. Kiri, and H. F. Edelhauser, "Effect of indocyanine green intraocular stain on human and rabbit corneal endothelial structure and viability. An in vitro study," J. Cataract Refract. Surg. 28, 1027-1033 (2002). [CrossRef] [PubMed]
  22. T. John, "Use of indocyanine green in deep lamellar endothelial keratoplasty," J. Cataract Refract. Surg. 29, 437-443 (2003). [CrossRef] [PubMed]
  23. L. Menabuoni, R. Pini, F. Rossi, I. Lenzetti, S. H. Yoo, and J.-M. Parel, "Laser-assisted corneal welding in cataract surgery: a retrospective study," J. Cataract Refract. Surg. (to be published).
  24. K. M. McNally-Heintzelman, "Laser Tissue Welding," in Biomedical Photonics Handbook, T. Vo-Dinh, ed., (CRC Press, 2003), Chap. 39, pp. 1-45.
  25. A. J. Welch and M. Van Germert, Optical-thermal response of laser-irradiated tissue (Plenum Press, 1995).
  26. A. Jain, Fundamentals of digital image processing (Prentice-Hall, 1989)
  27. M. N. Asiyo-Vogel, R. Brinkmann, H. Notbohm, R. Eggers, H. Lubatschowski, H. Laqua, and A. Vogel, "Histologic analysis of thermal effects of laser thermokeratoplasty and corneal ablation using Sirius-red polarization microscopy," J. Cataract Refract. Surg. 23, 515-26 (1997). [PubMed]

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