Optical sectioning microscopes with no moving parts using a micro-stripe array light emitting diode

doi:10.1364/OE.15.011196

Optics Express

Editor: C. Martijn de Sterke
Vol. 15, Iss. 18 — Sep. 3, 2007
pp: 11196–11206

Optical sectioning microscopes with no moving parts using a micro-stripe array light emitting diode

V. Poher, H. X. Zhang, G. T. Kennedy, C. Griffin, S. Oddos, E. Gu, D. S. Elson, M. Girkin, P. M. W. French, M. D. Dawson, and M. A. Neil »View Author Affiliations

Optics Express, Vol. 15, Issue 18, pp. 11196-11206 (2007)
http://dx.doi.org/10.1364/OE.15.011196

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Abstract
Article Info
References (18)
Cited By
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Abstract

We describe an optical sectioning microscopy system with no moving parts based on a micro-structured stripe-array light emitting diode (LED). By projecting arbitrary line or grid patterns onto the object, we are able to implement a variety of optical sectioning microscopy techniques such as grid-projection structured illumination and line scanning confocal microscopy, switching from one imaging technique to another without modifying the microscope setup. The micro-structured LED and driver are detailed and depth discrimination capabilities are measured and calculated. ©2007 Optical Society of America

OCIS Codes
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(180.1790) Microscopy : Confocal microscopy
(230.3670) Optical devices : Light-emitting diodes
(230.3990) Optical devices : Micro-optical devices

ToC Category:
Microscopy

History
Original Manuscript: May 15, 2007
Revised Manuscript: July 31, 2007
Manuscript Accepted: August 2, 2007
Published: August 21, 2007

Virtual Issues
Vol. 2, Iss. 10 Virtual Journal for Biomedical Optics

Citation
V. Poher, H. X. Zhang, G. T. Kennedy, C. Griffin, S. Oddos, E. Gu, D. S. Elson, M. Girkin, P. M. W. French, M. D. Dawson, and M. A. Neil, "Optical sectioning microscopes with no moving parts using a micro-stripe array light emitting diode," Opt. Express 15, 11196-11206 (2007)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-15-18-11196

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References

T. Wilson and Barry R. Masters, Confocal Microscopy, (Academic Press, San Diego, 1990).
W. B. Amos and J. G. White, "Direct view confocal imaging systems using a slit aperture," in Handbook of Biological Confocal Microscopy, J. B. Pawley (Plenum, New York, 1995), pp. 403-415.
C. J. R. Sheppard and X. Q. Mao, "Confocal microscopes with slit apertures," J. Mod. Opt. 25, 1169-1185 (1988). [CrossRef]
M. A. A. Neil, R. Juskaitis, and T. Wilson, "Method of obtaining optical sectioning by using structured light in a conventional microscope," Opt. Lett. 22, 1905-1907 (1997). [CrossRef]
OptiGrid, QiOptiq Imaging Solutions, http://www.qioptiqimaging.com>
ApoTome, Carl Zeiss MicroImaging GmbH, http://www.zeiss.com>
P. Herman, B. P. Maliwal, H.-J. Lin, and J. R. Lakowicz, "Frequency-domain fluorescence microscopy with the LED as a light source," J. Microsc. 203, 176-181 (2001). [CrossRef] [PubMed]
C. Moser, T. Mayr, and I. Klimant, "Filter cubes with built-in ultrabright light-emitting diodes as exchangeable excitation light sources in fluorescence microscopy," J. Microsc. 222, 135-140 (2006). [CrossRef] [PubMed]
O. Bormuth, J. Howard, and E. Schaffer, "LED illumination for video-enhanced DIC imaging of single microtubules," J. Microsc. 226, 1-5 (2007). [CrossRef] [PubMed]
S. X. Jin, J. Li, J. Y. Lin, and H. X. Jiang, "InGaN/GaN quantum well interconnected microdisk light emitting diodes," Appl. Phys. Lett. 77, 3236-3238 (2000).
H. W. Choi, C. W. Jeon, M. D. Dawson, P. R. Edwards, and R. W. Martin, "Fabrication and performance of parallel-addressed InGaN micro-LED arrays," IEEE Photon. Technol. Lett. 15, 510-512 (2003). [CrossRef]
C. W. Jeon, H. W. Choi, E. Gu, and M. D. Dawson, "High-density matrix-addressable AlInGaN-based 368-nm microarray light-emitting diodes," IEEE Photon. Technol. Lett. 16, 2421-2423 (2004). [CrossRef]
H. X. Zhang, E. Gu, C. W Jeon, Z. Gong, M. D. Dawson, M. A. A. Neil, and P. W. M. French, "Microstripe-array InGaN light-emitting diodes with individually addressable elements," IEEE Photon. Technol. Lett. 18, 1681-1683, (2006). [CrossRef]
M. Born, E. Wolf, Principles of Optics, (Pergamon Press, Oxford, 1975).
M. A. A. Neil, R. Juskaitis, and T. Wilson, "Real time 3D fluorescence microscopy by two beam interference illumination," Opt. Commun. 153, 1-4 (1998). [CrossRef]
L. H. Schaeffer, D. Schuster, and J. Schaffer, "Structured illumination microscopy: artefact analysis and reduction utilizing a parameter optimization approach," J. Microsc. 216, 165-174 (2004). [CrossRef]
P. A. Benedetti, V. Evangelista, D. Guidarini, and S. Vestri, "Electronic multiconfocal points microscopy," Three dimensional microscopy: Image acquisition and processing II, Proc. SPIE 2412, 56-62 (1995).
P. A. Stokseth, "Properties of a defocused optical system," J. Opt. Soc. Am. 59, 1314-1321 (1969). [CrossRef]

Appl. Phys. Lett.

S. X. Jin, J. Li, J. Y. Lin, and H. X. Jiang, "InGaN/GaN quantum well interconnected microdisk light emitting diodes," Appl. Phys. Lett. 77, 3236-3238 (2000).

IEEE Photon. Technol. Lett.

H. W. Choi, C. W. Jeon, M. D. Dawson, P. R. Edwards, and R. W. Martin, "Fabrication and performance of parallel-addressed InGaN micro-LED arrays," IEEE Photon. Technol. Lett. 15, 510-512 (2003). [CrossRef]
H. X. Zhang, E. Gu, C. W Jeon, Z. Gong, M. D. Dawson, M. A. A. Neil, and P. W. M. French, "Microstripe-array InGaN light-emitting diodes with individually addressable elements," IEEE Photon. Technol. Lett. 18, 1681-1683, (2006). [CrossRef]

J. Microsc.

L. H. Schaeffer, D. Schuster, and J. Schaffer, "Structured illumination microscopy: artefact analysis and reduction utilizing a parameter optimization approach," J. Microsc. 216, 165-174 (2004). [CrossRef]
P. Herman, B. P. Maliwal, H.-J. Lin, and J. R. Lakowicz, "Frequency-domain fluorescence microscopy with the LED as a light source," J. Microsc. 203, 176-181 (2001). [CrossRef] [PubMed]
C. Moser, T. Mayr, and I. Klimant, "Filter cubes with built-in ultrabright light-emitting diodes as exchangeable excitation light sources in fluorescence microscopy," J. Microsc. 222, 135-140 (2006). [CrossRef] [PubMed]
O. Bormuth, J. Howard, and E. Schaffer, "LED illumination for video-enhanced DIC imaging of single microtubules," J. Microsc. 226, 1-5 (2007). [CrossRef] [PubMed]

J. Mod. Opt.

C. J. R. Sheppard and X. Q. Mao, "Confocal microscopes with slit apertures," J. Mod. Opt. 25, 1169-1185 (1988). [CrossRef]

J. Opt. Soc. Am.

P. A. Stokseth, "Properties of a defocused optical system," J. Opt. Soc. Am. 59, 1314-1321 (1969). [CrossRef]

Opt. Commun.

M. A. A. Neil, R. Juskaitis, and T. Wilson, "Real time 3D fluorescence microscopy by two beam interference illumination," Opt. Commun. 153, 1-4 (1998). [CrossRef]

Opt. Lett.

M. A. A. Neil, R. Juskaitis, and T. Wilson, "Method of obtaining optical sectioning by using structured light in a conventional microscope," Opt. Lett. 22, 1905-1907 (1997). [CrossRef]

Photon. Technol. Lett.

C. W. Jeon, H. W. Choi, E. Gu, and M. D. Dawson, "High-density matrix-addressable AlInGaN-based 368-nm microarray light-emitting diodes," IEEE Photon. Technol. Lett. 16, 2421-2423 (2004). [CrossRef]

Proc. SPIE

P. A. Benedetti, V. Evangelista, D. Guidarini, and S. Vestri, "Electronic multiconfocal points microscopy," Three dimensional microscopy: Image acquisition and processing II, Proc. SPIE 2412, 56-62 (1995).

Other

M. Born, E. Wolf, Principles of Optics, (Pergamon Press, Oxford, 1975).
OptiGrid, QiOptiq Imaging Solutions, http://www.qioptiqimaging.com>
ApoTome, Carl Zeiss MicroImaging GmbH, http://www.zeiss.com>
T. Wilson and Barry R. Masters, Confocal Microscopy, (Academic Press, San Diego, 1990).
W. B. Amos and J. G. White, "Direct view confocal imaging systems using a slit aperture," in Handbook of Biological Confocal Microscopy, J. B. Pawley (Plenum, New York, 1995), pp. 403-415.

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