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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 15, Iss. 19 — Sep. 17, 2007
  • pp: 12005–12010

Thickness dependence of optical second harmonic generation in collagen fibrils

Shi-Wei Chu, Shih-Peng Tai, Ming-Che Chan, Chi-Kuang Sun, I-Ching Hsiao, Chi-Hung Lin, Yung-Chih Chen, and Bai-Ling Lin  »View Author Affiliations


Optics Express, Vol. 15, Issue 19, pp. 12005-12010 (2007)
http://dx.doi.org/10.1364/OE.15.012005


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Abstract

Simultaneous backward and forward second harmonic generations from isolated type-I collagen matrix are observed. Optical interference behaviors of these nonlinear optical signals are studied with accurately determined fibril thickness by an atomic force microscope. The nonlinear emission directions are strongly dependent on the coherent interaction within and between collagen fibrils. A linear relationship is obtained to estimate collagen fibril thickness with nanometer precision noninvasively by evaluating the forward/backward second harmonic generation ratio.

© 2007 Optical Society of America

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(180.6900) Microscopy : Three-dimensional microscopy
(190.4720) Nonlinear optics : Optical nonlinearities of condensed matter

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: July 23, 2007
Revised Manuscript: August 29, 2007
Manuscript Accepted: August 29, 2007
Published: September 5, 2007

Virtual Issues
Vol. 2, Iss. 10 Virtual Journal for Biomedical Optics

Citation
Shi-Wei Chu, Shih-Peng Tai, Ming-Che Chan, Chi-Kuang Sun, I-Ching Hsiao, Chi-Hung Lin, Yung-Chih Chen, and Bai-Ling Lin, "Thickness dependence of optical second harmonic generation in collagen fibrils," Opt. Express 15, 12005-12010 (2007)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-15-19-12005


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