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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 15, Iss. 19 — Sep. 17, 2007
  • pp: 12286–12295

Estimation of helical angles of myosin and collagen by second harmonic generation imaging microscopy

François Tiaho, Gaëlle Recher, and Denis Rouède  »View Author Affiliations

Optics Express, Vol. 15, Issue 19, pp. 12286-12295 (2007)

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We performed Second Harmonic Generation (SHG) imaging microscopy of endogeneous myosin-rich and collagen-rich tissues in amphibian and mammals. We determined the relative components of the macroscopic susceptibility tensor χ(2) from polarization dependence of SHG intensity. The effective orientation angle θe of the harmonophores has been determined for each protein. For myosin we found θe≈62° and this value was unchanged during myofibrillogenesis. It was also independent of the animal species (xenopus, dog and human). For collagen we found θe≈49° for both type I- and type III- rich tissues. From these results we localized the source of SHG along the single helix of both myosin and collagen.

© 2007 Optical Society of America

OCIS Codes
(170.0180) Medical optics and biotechnology : Microscopy
(180.0180) Microscopy : Microscopy
(190.4160) Nonlinear optics : Multiharmonic generation

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: July 26, 2007
Revised Manuscript: August 27, 2007
Manuscript Accepted: August 30, 2007
Published: September 12, 2007

Virtual Issues
Vol. 2, Iss. 10 Virtual Journal for Biomedical Optics

François Tiaho, Gaëlle Recher, and Denis Rouède, "Estimation of helical angles of myosin and collagen by second harmonic generation imaging microscopy," Opt. Express 15, 12286-12295 (2007)

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