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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 15, Iss. 20 — Oct. 1, 2007
  • pp: 12583–12594

Development of a synchronous fluorescence imaging system and data analysis methods

Quan Liu, Kui Chen, Matthew Martin, Alan Wintenberg, Roberto Lenarduzzi, Masoud Panjehpour, Bergein F. Overholt, and Tuan Vo-Dinh  »View Author Affiliations

Optics Express, Vol. 15, Issue 20, pp. 12583-12594 (2007)

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Although conventional autofluorescence spectroscopy, in which fluorescence emission spectra are recorded for fixed excitation wavelengths, has demonstrated good performance in tissue diagnosis, it suffers from prolonged data acquisition time and broad-band fluorescence features. Synchronous spectroscopy has been proposed to overcome the limitations of conventional fluorescence spectroscopy but has not been applied to imaging for tissue diagnosis in vivo. Our group has developed a synchronous fluorescence imaging system to combine the great diagnostic potential of synchronous spectroscopy and the large field of view of imaging for cancer diagnosis. This system has been tested in a mouse skin model to capture synchronous fluorescence images. A simple discriminant analysis method and a more complicated multi-variate statistical method have been developed to generate a single diagnostic image from a large number of raw fluorescence images. Moreover, it was demonstrated that the diagnostic image generated from synchronous data is comparable to that generated from full spectral data in classification accuracy.

© 2007 Optical Society of America

OCIS Codes
(100.2000) Image processing : Digital image processing
(100.2960) Image processing : Image analysis
(110.2350) Imaging systems : Fiber optics imaging
(120.6200) Instrumentation, measurement, and metrology : Spectrometers and spectroscopic instrumentation
(170.2150) Medical optics and biotechnology : Endoscopic imaging
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence

ToC Category:
Image Processing

Original Manuscript: June 21, 2007
Revised Manuscript: September 12, 2007
Manuscript Accepted: September 13, 2007
Published: September 17, 2007

Virtual Issues
Vol. 2, Iss. 11 Virtual Journal for Biomedical Optics

Quan Liu, Kui Chen, Matthew Martin, Alan Wintenberg, Roberto Lenarduzzi, Masoud Panjehpour, Bergein F. Overholt, and Tuan Vo-Dinh, "Development of a synchronous fluorescence imaging system and data analysis methods," Opt. Express 15, 12583-12594 (2007)

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