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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 15, Iss. 20 — Oct. 1, 2007
  • pp: 12708–12716

NIR Raman spectroscopic investigation of single mitochondria trapped by optical tweezers

Haiyang Tang, Huilu Yao, Guiwen Wang, Yun Wang, Yong-qing Li, and Meifu Feng  »View Author Affiliations

Optics Express, Vol. 15, Issue 20, pp. 12708-12716 (2007)

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Raman spectroscopy is a vibration spectroscopic technique that has been widely used to probe biochemical changes of biological sample such as tumor tissue, blood cells, bacteria and yeast. Here, we applied near-infrared Raman spectroscopy to analyze the chemical composition changes of intact or swollen mitochondria induced by calcium ions. We used a confocal Laser Tweezers Raman Spectroscopy (LTRS) system that combined optical trapping and near infrared Raman spectroscopy to confine a single mitochondrion and consequently measure its Raman spectra following the addition of calcium ion solution. We analyzed Raman spectra of mitochondria isolated from rat liver, heart muscle and kidney, respectively. The major Raman peaks at 1654, 1602, 1446, 1301 and 1226 cm−1 were observed from individual intact mitochondria. We examined the differences in near infrared spectra between intact and Ca2+ damaged mitochondria. We found that after the exposure of the intact mitochondria to the 100 μM Ca2+ solution the band of 1602 cm−1 decreased very rapidly in the first period and then disappeared after 30minutes, while the intensities of the phospholipids and protein bands changed slowly in the first period and then suddenly disappeared, corresponding to the Ca2+ induced swelling process. These results demonstrate the potential of LTRS technique as a valuable tool for the study of bioactivity and molecular composition of mitochondria.

© 2007 Optical Society of America

OCIS Codes
(170.1530) Medical optics and biotechnology : Cell analysis
(170.5660) Medical optics and biotechnology : Raman spectroscopy

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: July 5, 2007
Revised Manuscript: August 31, 2007
Manuscript Accepted: September 4, 2007
Published: September 20, 2007

Virtual Issues
Vol. 2, Iss. 11 Virtual Journal for Biomedical Optics

Haiyang Tang, Huilu Yao, Guiwen Wang, Yun Wang, Yong-qing Li, and Meifu Feng, "NIR Raman spectroscopic investigation of single mitochondria trapped by optical tweezers," Opt. Express 15, 12708-12716 (2007)

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