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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 15, Iss. 21 — Oct. 17, 2007
  • pp: 13597–13606

Photon statistics in single molecule orientational imaging

Matthew R. Foreman, Sherif S. Sherif, and Peter Türük  »View Author Affiliations


Optics Express, Vol. 15, Issue 21, pp. 13597-13606 (2007)
http://dx.doi.org/10.1364/OE.15.013597


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Abstract

Optical techniques in single molecule imaging rely heavily on photon counting for data acquisition. Extraction of information from the recorded readings is often done by means of statistical signal processing, however this requires a full knowledge of the photoelectron statistics. In addition to counting statistics we include a specific form of random signal variations namely reorientational dynamics, or wobble to derive the general probability density function of the number of detected photons. The relative importance of the two factors is dependent upon the total number of photons in the system and results are given in all regimes.

© 2007 Optical Society of America

OCIS Codes
(000.5490) General : Probability theory, stochastic processes, and statistics
(110.4280) Imaging systems : Noise in imaging systems
(180.2520) Microscopy : Fluorescence microscopy

ToC Category:
Imaging Systems

History
Original Manuscript: May 17, 2007
Revised Manuscript: September 28, 2007
Manuscript Accepted: September 28, 2007
Published: October 2, 2007

Virtual Issues
Vol. 2, Iss. 11 Virtual Journal for Biomedical Optics

Citation
Matthew R. Foreman, Sherif S. Sherif, and Peter Török, "Photon statistics in single molecule orientational imaging," Opt. Express 15, 13597-13606 (2007)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-15-21-13597


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