OSA's Digital Library

Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 15, Iss. 21 — Oct. 17, 2007
  • pp: 13640–13648

Imaging in digital holographic microscopy

Shan Shan Kou and Colin J. R. Sheppard  »View Author Affiliations

Optics Express, Vol. 15, Issue 21, pp. 13640-13648 (2007)

View Full Text Article

Enhanced HTML    Acrobat PDF (918 KB)

Browse Journals / Lookup Meetings

Browse by Journal and Year


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools



We present a theoretical formalism for three dimensional (3D) imaging properties of digital holographic microscopy (DHM). Through frequency analysis and visualization of its 3D optical transfer function, an assessment of the imaging behavior of DHM is given. The results are compared with those from other types of interference microscopy. Digital holographic microscopy does not result in true 3D imaging. The main advantage of holographic microscopy lies in its quick acquisition of a single 2D image. Full 3D imaging can be obtained with DHM using a broad-band source or tomographic reconstruction.

© 2007 Optical Society of America

OCIS Codes
(090.0090) Holography : Holography
(110.2990) Imaging systems : Image formation theory
(180.6900) Microscopy : Three-dimensional microscopy

ToC Category:

Original Manuscript: June 12, 2007
Revised Manuscript: September 25, 2007
Manuscript Accepted: September 28, 2007
Published: October 3, 2007

Virtual Issues
Vol. 2, Iss. 11 Virtual Journal for Biomedical Optics

Shan S. Kou and Colin J. Sheppard, "Imaging in digital holographic microscopy," Opt. Express 15, 13640-13648 (2007)

Sort:  Author  |  Year  |  Journal  |  Reset  


  1. D. Gabor, "A new microscope principle," Nature 161,777-778 (1948). [CrossRef] [PubMed]
  2. U. Schnars and W. Juptner, "Direct recording of holograms by a CCD target and numerical reconstruction," Appl. Opt. 33,179-181 (1994). [CrossRef] [PubMed]
  3. P. Marquet, B. Rappaz, P. Magistretti, E. Cuche, Y. Emery, T. Colomb, and C. Depeursinge,"Digital holographic microscopy: a noninvasive contrast imaging technique allowing quantitative visualization of living cells with subwavelength axial accuracy," Opt. Lett. 30,468-470 (2005). [CrossRef] [PubMed]
  4. B. Kemper, D. Carl, J. Schnekenburger, I. Bredebusch, M. Schafer, W. Domschke, and G. von Bally, "Investigation of living pancreas tumor cells by digital holographic microscopy," J. Biomed. Opt. 11,034005 (2006). [CrossRef]
  5. A. Stern and B. Javidi,"Theoretical analysis of three-dimensional imaging and recognition of micro-organisms with a single-exposure on-line holographic microscope," J. Opt. Soc. Am. 24,163-168 (2007). [CrossRef]
  6. I. Yamaguchi and T. Zhang,"Phase-shifting digital holography," Opt. Lett. 22,1268-1270 (1997). [CrossRef] [PubMed]
  7. P. Ferraro, S. De Nicola, A. Finizio, G. Coppola, S. Grilli, C. Magro, and G. Pierattini,"Compensation of the inherent wave front curvature in digital holographic coherent microscopy for quantitative phase-contrast imaging," Appl. Opt. 42,1938-1946 (2003). [CrossRef] [PubMed]
  8. S. A. Alexandrov, T. R. Hillman, T. Gutzler, and D. D. Sampson, "Synthetic aperture fourier holographic optical microscopy," Phys. Rev. Lett. 97,168102 (2006). [CrossRef] [PubMed]
  9. T. Turpin, L. Gesell, J. Lapides, and C. Price, "Theory of the Synthetic Aperture Microscope," Proc. SPIE 2566,230-240 (1995). [CrossRef]
  10. R. Chmelik, "Three-dimensional scalar imaging in high-aperture low-coherence interference and holographic microscopes," J. Mod. Optic. 53,2673-2689 (2006). [CrossRef]
  11. C. W. McCutchen, "Generalized aperture and the three-dimensional diffraction image," J. Opt. Soc. Am. 54,240-244 (1964). [CrossRef]
  12. C. J. R. Sheppard and K. G. Larkin, "Vectorial pupil functions and vectorial transfer functions," Optik 107,79-87 (1997).
  13. E. Wolf, "Three-dimensional structure determination of semi-transparent objects from holographic data," Opt. Commun. 1,153-156 (1969). [CrossRef]
  14. C. J. R. Sheppard, "The spatial frequency cut-off in three-dimensional imaging," Optik 72,131-133 (1986).
  15. B. R. Frieden, "Optical Transfer of the Three-Dimensional Object," J. Opt. Soc. Am. 57,56-66 (1967). [CrossRef]
  16. C. J. R. Sheppard and M. Gu, "The significance of 3-D transfer functions in confocal scanning microscopy," J. Microsc. 165,377-390 (1991). [CrossRef]
  17. D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, "Optical Coherence Tomography," Science 254,1178-1181 (1991). [CrossRef] [PubMed]
  18. D. K. Hamilton and C. J. R. Sheppard, "A confocal interference microscope," Opt. Acta 29,1573-1577 (1982). [CrossRef]
  19. C. J. R. Sheppard, M. Roy, and M. D. Sharma, "Image formation in low-coherence and confocal interference microscopes," Appl. Opt. 43,1493-1502 (2004). [CrossRef] [PubMed]
  20. M. Davidson, K. Kaufman, and I. Mazor, "The Coherence Probe Microscope," Solid State Technol. 30,57-59 (1987).
  21. C. J. R. Sheppard and T. Wilson, "Fourier imaging of phase information in conventional and scanning microscopes," Philos. Tr. R. Soc. S-A 295,513-536 (1980). [CrossRef]
  22. M. Born and E. Wolf, Principles of Optics, Cambridge University Press, 7th ed. 2005.
  23. C. J. R. Sheppard and M. Gu, "Imaging by high-aperture optical system," J. Mod. Optic. 40,1631-1651 (1993). [CrossRef]
  24. L. Mart’?nez-Le’on, G. Pedrini, andW. Osten, "Applications of short-coherence digital holography in microscopy," Appl. Opt. 44,3977-3984 (2005). [CrossRef] [PubMed]
  25. A. J. Devaney, "A filtered back propagation algorithm for diffraction tomography," Ultrason. Imaging 4,336-350 (1982). [CrossRef] [PubMed]
  26. V. Lauer, "New approach to optical diffraction tomography yielding a vector equation of diffraction tomography and a novel tomographic microscope," J. Microsc. 205,165-176 (2002). [CrossRef] [PubMed]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited