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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 15, Iss. 21 — Oct. 17, 2007
  • pp: 14184–14193

Label-free optical imaging of mitochondria in live cells

David Lasne, Gerhard. A. Blab, Francesca De Giorgi, François Ichas, Brahim Lounis, and Laurent Cognet  »View Author Affiliations

Optics Express, Vol. 15, Issue 21, pp. 14184-14193 (2007)

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The far-field optical imaging of mitochondria of live cells without the use of any label is demonstrated. It uses a highly sensitive photothermal method and has a resolution comparable to confocal fluorescence setups. The morphological states of mitochondria were followed under different physiological treatments, and the role of cytochrome c was ruled out as the main origin of the photothermal signals. This label free optical method provides a high contrast imaging of live mitochondria and should find many applications in biosciences.

© 2007 Optical Society of America

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.4580) Medical optics and biotechnology : Optical diagnostics for medicine
(180.3170) Microscopy : Interference microscopy
(190.4870) Nonlinear optics : Photothermal effects

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: July 17, 2007
Revised Manuscript: September 7, 2007
Manuscript Accepted: September 11, 2007
Published: October 12, 2007

Virtual Issues
Vol. 2, Iss. 11 Virtual Journal for Biomedical Optics

David Lasne, Gerhard A. Blab, Francesca De Giorgi, François Ichas, Brahim Lounis, and Laurent Cognet, "Label-free optical imaging of mitochondria in live cells," Opt. Express 15, 14184-14193 (2007)

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