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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 15, Iss. 23 — Nov. 12, 2007
  • pp: 15101–15114

Sidestream Dark Field (SDF) imaging: a novel stroboscopic LED ring-based imaging modality for clinical assessment of the microcirculation.

P. T. Goedhart, M. Khalilzada, R. Bezemer, J. Merza, and C. Ince  »View Author Affiliations

Optics Express, Vol. 15, Issue 23, pp. 15101-15114 (2007)

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Sidestream Dark Field (SDF) imaging, a stroboscopic LED ring-based imaging modality, is introduced for clinical observation of the microcirculation. SDF imaging is validated by comparison to Orthogonal Polarization Spectral imaging. Nailfold capillary diameters and red blood cell velocities were measured using both techniques and equal quantitative results were obtained. An image quality system was developed to quantitatively compare the quality of sublingually-acquired microcirculatory images using OPS and SDF imaging. Venular contrast, sharpness, and quality were shown to be comparable for OPS and SDF imaging. However, capillary contrast and quality were shown to be significantly higher using SDF imaging. Venular granularity, in addition, was more clearly observable using SDF imaging.

© 2007 Optical Society of America

OCIS Codes
(110.0180) Imaging systems : Microscopy
(170.0110) Medical optics and biotechnology : Imaging systems
(170.5380) Medical optics and biotechnology : Physiology

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: August 7, 2007
Revised Manuscript: October 11, 2007
Manuscript Accepted: October 25, 2007
Published: October 31, 2007

Virtual Issues
Vol. 2, Iss. 12 Virtual Journal for Biomedical Optics

P. T. Goedhart, M. Khalilzada, R. Bezemer, J. Merza, and C. Ince, "Sidestream Dark Field (SDF) imaging: a novel stroboscopic LED ring-based imaging modality for clinical assessment of the microcirculation," Opt. Express 15, 15101-15114 (2007)

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  1. B. Fagrell and A. Bollinger, eds., Clinical Capillaroscopy (Hogrefe & Huber, 1990).
  2. B. Fagrell, A. Fronek, and M. Intaglietta, "A microscope-television system for studying flow velocity in human skin capillaries," Am. J. Physiol. Heart Circ. Physiol. 233, 318-321 (1977).
  3. D. W. Slaaf, G. J. Tangelder, R. S. Reneman, K. Jager, and A. Bollinger, "A versatile incident illuminator for intravital microscopy," Int. J. Microcirc. Clin. Exp. 6, 391-397 (1987). [PubMed]
  4. W. Groner, J. W. Winkelman, A. G. Harris, C. Ince, G. J. Bouma, K. Messmer, and R. G. Nadeau, "Orthogonal polarization spectral imaging: a new method for study of the microcirculation," Nat. Med. 5, 1209-1212 (1999). [CrossRef] [PubMed]
  5. S. Trzeciak, R. P. Dellinger, J. E. Parrillo, M. Guglielmi, J. Bajaj, N. L. Abate, R. C. Arnold, S. Colilla, S. Zanotti, and S. M. Hollenberg, "Early microcirculatory perfusion derangements in patients with severe sepsis and septic shock: relationship to hemodynamics, oxygen transport, and survival," Ann. Emerg. Med. 49, 88-98 (2007). [CrossRef]
  6. P. E. Spronk, C. Ince, M. J. Gardien, K. R. Mathura, H. M. Oudemans-van Straaten, and D. F. Zandstra, "Nitroglycerin in septic shock after intravascular volume resuscitation," Lancet 360, 1395-1396 (2002). [CrossRef] [PubMed]
  7. Y. Sakr, M. J. Dubois, D. de Backer, J. Creteur, and J. L. Vincent, "Persistent microcirculatory alterations are associated with organ failure and death in patients with septic shock," Crit. Care Med. 32, 1825-1831 (2004). [CrossRef] [PubMed]
  8. D. de Backer, J. Creteur, M. J. Dubois, Y. Sakr, and J. L. Vincent, "Microvascular alterations in patients with acute severe heart failure and cardiogenic shock," Am. Heart J. 147, 91-99 (2004). [CrossRef]
  9. A. Dubin, E. V. S. Kanoore, M. Pozo, G. Murias, C. Canullán, E. Martins, G. Ferrara, H. Canales, M. Laporte, E. Estenssoro, and C. Ince, "Persistent villi hypoperfusion explains intramucosal acidosis in sheep endotoxemia," Crit. Care Med. (in press).
  10. E. C. Boerma, P. H. J. van der Voort, P. E. Spronk, and C. Ince, "Relationship between sublingual and intestinal microcirculatory perfusion in patients with abdominal sepsis," Crit. Care Med. 35, 1055-1060 (2007). [CrossRef] [PubMed]
  11. J. A. H. Lindeboom, K. R. Mathura, I. H. Aartman, F. Kroon, and C. Ince, "The influence of the application of platelet enriched plasmas in oral mucosal wound healing," Clin. Oral Impl. Res. 18, 133-139 (2007). [CrossRef]
  12. A. M. Dondorp, C. Ince, P. Tipmanee, J. Hanson, A. van Kuijen, M. A. Faiz, M. R. Rahman, M. Hasan, E. Bin Yunus, A. Ghose, R. Ruangveerayut, D. Limmathurotsakul, K. R. Mathura, N. J. White, and N. P. J. Day, "Direct in-vivo assessment of microcirculatory dysfunction in severe falciparum malaria," J. Infect. Dis. (in press).
  13. F. Pennings, G. J. Bouma, and C. Ince, "Direct observation of the human cerebral microcirculation during aneurysm surgery reveals increased arteriolar contractility," Stroke 35, 1284-1288 (2004). [CrossRef] [PubMed]
  14. K. R. Mathura, G. J. Bouma, and C. Ince, "Abnormal microcirculation in brain tumors during surgery," Lancet 358, 1698-1699 (2001). [CrossRef] [PubMed]
  15. C. Ince, "The microcirculation is the motor of sepsis," Crit. Care 9, Suppl. 4, 13-19 (2005).
  16. D. de Backer, J. Creteur, J. C. Preiser, M. J. Dubois, and J. L. Vincent, "Microvascular blood flow is altered in patients with sepsis," Am. J. Respir. Crit. Care Med. 166, 98-104 (2002). [CrossRef] [PubMed]
  17. H. A. Lehr, F. Bittinger, and C. J. Kirkpatrick, "Microcirculatory dysfunction in sepsis: a pathogenetic basis for therapy?" J. Pathol. 190, 373-386 (2000). [CrossRef] [PubMed]
  18. D. R. Dantzker, "Adequacy of tissue oxygenation," Crit. Care Med. 21, Suppl. 2, 40-43 (1993). [CrossRef]
  19. J. Lindert, J. Werner, M. Redlin, H. Kuppe, H. Habazettl, and A. R. Pries, "OPS imaging of human microcirculation: a short technical report," J. Vasc. Res. 39, 368-372 (2002). [CrossRef] [PubMed]
  20. V. Cerný, Z. Turek, and R. Pařízková, "Orthogonal Polarization Spectral imaging: a review," Physiol. Res. 56 (2007).
  21. M. Heger, J. F. Beek, K. Stenback, D. J. Faber, and M. J.C. van Gemert, "Darkfield orthogonal polarized spectral imaging for studying endovascular laser-tissue interactions in vivo - a preliminary study," Opt. Express 13, 702-715 (2005). [CrossRef] [PubMed]
  22. C. Ince, "Sidestream dark field imaging: an improved technique to observe sublingual microcirculation," Crit. Care 9, Suppl. 1, 72 (2005).
  23. Z. Turek, V. Cerný, and R. Pařízková, "Noninvasive in vivo assessment of the skeletal muscle and small intestine serous surface microcirculation in rat: sidestream dark-field (SDF) imaging," Physiol. Res. 56 (2007).
  24. Visible and near infrared absorption spectra of human and animal haemoglobin determination and application, W. G. Zijlstra, A. Buursma, and O. W. van Assendelft, eds. (Brill Academic Publishers, 2000).
  25. D. de Backer, S. Hollenberg, C. Boerma, P. T. Goedhart, G. Büchele, G. Ospina-Tascon, I. Dobbe, and C. Ince, "How to evaluate the microcirculation? Report of a round table conference," Crit. Care (in press).
  26. K. R. Mathura, K. C. Vollebergt, K. Boer, J. C. de Graaf, D. T. Ubbink, and C. Ince, "Comparison of OPS imaging and conventional capillary microscopy to study the human microcirculation," J. Appl. Physiol. 91, 74-78 (2001). [PubMed]
  27. T. K. Bergersen, M. Eriksen, and L. Walloe, "Effect of local warming on hand and finger artery blood velocities," Am. J. Physiol. Regul. Integr. Comp. Physiol. 269, 325-330 (1995).
  28. J. M. Bland and D. G. Altman, "Statistical methods for assessing agreement between two methods of clinical measurement," Lancet 1, 307-310 (1986). [CrossRef] [PubMed]
  29. J. M. Bland and D. G. Altman, "Comparing methods of measurement: why plotting difference against standard method is misleading," Lancet 346, 1085-1087 (1995). [CrossRef] [PubMed]
  30. C. G. Ellis, M. L. Ellsworth, R. N. Pittman, and W. L. Burgess, "Application of image analysis for evaluation of red blood cell dynamics in capillaries," Microvasc. Res. 44, 214-225 (1992). [CrossRef] [PubMed]
  31. J. G. G. Dobbe, G. J. Streekstra, B. Atasever, R. van Zijderveld, and C. Ince, "Measurement of functional microcirculatory geometry and velocity distributions using automated image analysis," submitted toMicrocirculation.
  32. K. R. Mathura, L. Alic, and C. Ince, "Initial clinical experience with OPS imaging for observation of the human microcirculation," in: Yearbook of intensive care and emergency medicine, J. L. Vincent, ed. (Springer, 2001).

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