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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 15, Iss. 24 — Nov. 26, 2007
  • pp: 16029–16034

Calculation of spherical red blood cell deformation in a dual-beam optical stretcher

Paul B. Bareil, Yunlong Sheng, Yin-Quan Chen, and Arthur Chiou  »View Author Affiliations


Optics Express, Vol. 15, Issue 24, pp. 16029-16034 (2007)
http://dx.doi.org/10.1364/OE.15.016029


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Abstract

We present a numerical method based on the linear elastic membrane theory to compute the morphological deformation of a spherical cell from the photonics stress distribution over the cellular membrane. The method is applied to fit the experimental data for deformation of a spherical human red blood cell trapped and stretched in a fiber-optical dual-beam trap with a single fitting parameter Eh where E is the Young’s modulus of elasticity and h is the thickness of the cell membrane. We obtained Eh=(20±2)µNm-1 which is comparable to results reported earlier. This numerical method can be applied in general experimental conditions.

© 2007 Optical Society of America

OCIS Codes
(140.7010) Lasers and laser optics : Laser trapping
(170.1420) Medical optics and biotechnology : Biology
(170.1530) Medical optics and biotechnology : Cell analysis
(170.4520) Medical optics and biotechnology : Optical confinement and manipulation
(350.4855) Other areas of optics : Optical tweezers or optical manipulation

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: August 17, 2007
Revised Manuscript: November 6, 2007
Manuscript Accepted: November 6, 2007
Published: November 19, 2007

Virtual Issues
Vol. 2, Iss. 12 Virtual Journal for Biomedical Optics

Citation
Paul B. Bareil, Yunlong Sheng, Yin-Quan Chen, and Arthur Chiou, "Calculation of spherical red blood cell deformation in a dual-beam optical stretcher," Opt. Express 15, 16029-16034 (2007)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-15-24-16029


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References

  1. J. Guck, R. Ananthakrishnan, H. Mahmood, T. J. Moon, C.C. Cunningham, and J. Käs, "The optical stretcher: a novel laser tool to micromanipulate cells," Biophys. J. 81,767-784 (2001). [CrossRef] [PubMed]
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  8. M. Dao, J. Li, S. Suresh, "Molecularly based analysis of deformation of spectrin network and human erythrocyte," Materials Science and Engineering 26,1232 - 1244 (2006). [CrossRef]

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