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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 15, Iss. 25 — Dec. 10, 2007
  • pp: 16413–16423

Subcellular-resolution molecular imaging within living tissue by fiber microendoscopy

Timothy J. Muldoon, Mark C. Pierce, Dawn L. Nida, Michelle D. Williams, Ann Gillenwater, and Rebecca Richards-Kortum  »View Author Affiliations

Optics Express, Vol. 15, Issue 25, pp. 16413-16423 (2007)

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Conventional histopathology involves sampling, sectioning and staining of tissue specimens prior to microscopic evaluation, and provides diagnostic information at a single location and point in time. In vivo microscopy and molecular-targeted optical labeling are two rapidly developing fields, which together have the potential to provide anatomical and functional indications of disease by staining and imaging tissue in situ. To address the need for high-resolution imaging instrumentation, we have developed a compact, robust, and inexpensive fiber-optic microendoscopy system based around wide-field LED illumination, a flexible 1 mm diameter fiber-optic bundle, and a color CCD camera. We demonstrate the subcellular resolution imaging capabilities of the system through a series of experiments, beginning with simultaneous imaging of three different cancer cell lines in culture, each targeted with a distinct fluorescent label. We used the narrow diameter probe to access subcutaneous tumors in an in vivo murine model, allowing direct comparison of microendoscopy images with macroscopic images and histopathology. A surgically resected tissue specimen from the human oral cavity was imaged across the clinical margin, demonstrating qualitative and quantitative distinction between normal and cancerous tissue based on sub-cellular image features. Finally, the fiber-optic microendoscope was used on topically-stained normal human oral mucosa in vivo, resolving epithelial cell nuclei and membranes in real-time fluorescence images. Our results demonstrate that this imaging system can potentially complement conventional diagnostic techniques, and support efforts to translate emerging molecular-diagnostic and therapeutic agents into clinical use.

© 2007 Optical Society of America

OCIS Codes
(170.2150) Medical optics and biotechnology : Endoscopic imaging
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.4580) Medical optics and biotechnology : Optical diagnostics for medicine

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: October 18, 2007
Revised Manuscript: November 14, 2007
Manuscript Accepted: November 21, 2007
Published: November 27, 2007

Virtual Issues
Vol. 3, Iss. 1 Virtual Journal for Biomedical Optics

Timothy J. Muldoon, Mark C. Pierce, Dawn L. Nida, Michelle D. Williams, Ann Gillenwater, and Rebecca Richards-Kortum, "Subcellular-resolution molecular imaging within living tissue by fiber microendoscopy," Opt. Express 15, 16413-16423 (2007)

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