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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 15, Iss. 25 — Dec. 10, 2007
  • pp: 16561–16580

Discrimination of granulocyte subtypes from light scattering: theoretical analysis using a granulated sphere model

Maxim A. Yurkin, Konstantin A. Semyanov, Valeri P. Maltsev, and Alfons G. Hoekstra  »View Author Affiliations


Optics Express, Vol. 15, Issue 25, pp. 16561-16580 (2007)
http://dx.doi.org/10.1364/OE.15.016561


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Abstract

We perform extensive simulations of light scattering by a granulated sphere in the size and refractive index range of human granulated leucocytes using the discrete dipole approximation. We calculate total and depolarized side scattering signals as a function of the size and refractive indices of cell and granules, and the granule volume fraction. Using typical parameters derived from the literature data on granulocyte morphology, we show that differences between experimentally measured signals of two granulocyte subtypes can be explained solely by the difference in their granule sizes. Moreover, the calculated depolarization ratio quantitatively agrees with experimental results. We also use the Rayleigh-Debye-Gans approximation and its second order extension to derive analytical expressions for side scattering signals. These expressions qualitatively describe the scaling of signals with varying model parameters obtained by rigorous simulations, and even lead to quantitative agreement in some cases. Finally, we show and discuss the dependence of extinction efficiency and asymmetry parameter on size and volume fraction of granules.

© 2007 Optical Society of America

OCIS Codes
(000.4430) General : Numerical approximation and analysis
(170.1530) Medical optics and biotechnology : Cell analysis
(290.4210) Scattering : Multiple scattering
(290.5850) Scattering : Scattering, particles
(290.5855) Scattering : Scattering, polarization

ToC Category:
Scattering

History
Original Manuscript: October 10, 2007
Revised Manuscript: November 12, 2007
Manuscript Accepted: November 12, 2007
Published: November 29, 2007

Virtual Issues
Vol. 3, Iss. 1 Virtual Journal for Biomedical Optics

Citation
Maxim A. Yurkin, Konstantin A. Semyanov, Valeri P. Maltsev, and Alfons G. Hoekstra, "Discrimination of granulocyte subtypes from light scattering: theoretical analysis using a granulated sphere model," Opt. Express 15, 16561-16580 (2007)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-15-25-16561


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