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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 15, Iss. 5 — Mar. 5, 2007
  • pp: 2244–2250

Fluorescence incoherent color holography

Joseph Rosen and Gary Brooker  »View Author Affiliations

Optics Express, Vol. 15, Issue 5, pp. 2244-2250 (2007)

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We present a new imaging method to record multicolor digital holograms from objects emitting fluorescent light. The fluorescent light specific to the emission wavelength of various fluorescent dyes after excitation of three dimensional (3D) objects is recorded on a digital monochrome camera after reflection from a diffractive optical element (DOE). For each wavelength of fluorescent emission, the camera sequentially records three holograms reflected from the DOE, each with a different phase factor of the DOE’s function. The three holograms are superposed in a computer to create a complex valued Fresnel hologram of each fluorescent emission. The holograms for each fluorescent color are further combined in a computer to produce a multicolored fluorescence hologram and 3D color image.

© 2007 Optical Society of America

OCIS Codes
(090.1760) Holography : Computer holography
(100.6890) Image processing : Three-dimensional image processing

ToC Category:

Original Manuscript: January 22, 2007
Revised Manuscript: February 21, 2007
Manuscript Accepted: February 26, 2007
Published: March 5, 2007

Joseph Rosen and Gary Brooker, "Fluorescence incoherent color holography," Opt. Express 15, 2244-2250 (2007)

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Fig. 1. Fig. 2.

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