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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 15, Iss. 5 — Mar. 5, 2007
  • pp: 2454–2458

Two-photon fluorescence imaging with a pulse source based on a 980-nm gain-switched laser diode

Kenji Taira, Takeshi Hashimoto, and Hiroyuki Yokoyama  »View Author Affiliations

Optics Express, Vol. 15, Issue 5, pp. 2454-2458 (2007)

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We developed a compact, high-peak-power picosecond pulse source based on a gain-switched laser diode with an emission wavelength of 980 nm and successfully demonstrated two-photon fluorescence imaging. The pulse source consisted of the gain-switched laser diode, a pulse compressor, a pulse reshaper, and optical amplifiers, all connected by single-mode fibers. The optical pulse generated by the pulse source had a pulse width of 3.5 ps, an average power of 35 mW, and kilowatt-level peak power at a repetition rate of 10 MHz. Alexa488-stained Glomeruli and convoluted tubules of a mouse kidney section were observed by two-photon imaging with the developed pulse source.

© 2007 Optical Society of America

OCIS Codes
(140.5960) Lasers and laser optics : Semiconductor lasers
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(190.4180) Nonlinear optics : Multiphoton processes
(320.5390) Ultrafast optics : Picosecond phenomena
(320.5550) Ultrafast optics : Pulses

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: December 21, 2006
Revised Manuscript: February 15, 2007
Manuscript Accepted: February 15, 2007
Published: March 5, 2007

Virtual Issues
Vol. 2, Iss. 4 Virtual Journal for Biomedical Optics

Kenji Taira, Takeshi Hashimoto, and Hiroyuki Yokoyama, "Two-photon fluorescence imaging with a pulse source based on a 980-nm gain-switched laser diode," Opt. Express 15, 2454-2458 (2007)

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