OSA's Digital Library

Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 15, Iss. 5 — Mar. 5, 2007
  • pp: 2454–2458

Two-photon fluorescence imaging with a pulse source based on a 980-nm gain-switched laser diode

Kenji Taira, Takeshi Hashimoto, and Hiroyuki Yokoyama  »View Author Affiliations


Optics Express, Vol. 15, Issue 5, pp. 2454-2458 (2007)
http://dx.doi.org/10.1364/OE.15.002454


View Full Text Article

Enhanced HTML    Acrobat PDF (421 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

We developed a compact, high-peak-power picosecond pulse source based on a gain-switched laser diode with an emission wavelength of 980 nm and successfully demonstrated two-photon fluorescence imaging. The pulse source consisted of the gain-switched laser diode, a pulse compressor, a pulse reshaper, and optical amplifiers, all connected by single-mode fibers. The optical pulse generated by the pulse source had a pulse width of 3.5 ps, an average power of 35 mW, and kilowatt-level peak power at a repetition rate of 10 MHz. Alexa488-stained Glomeruli and convoluted tubules of a mouse kidney section were observed by two-photon imaging with the developed pulse source.

© 2007 Optical Society of America

OCIS Codes
(140.5960) Lasers and laser optics : Semiconductor lasers
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(190.4180) Nonlinear optics : Multiphoton processes
(320.5390) Ultrafast optics : Picosecond phenomena
(320.5550) Ultrafast optics : Pulses

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: December 21, 2006
Revised Manuscript: February 15, 2007
Manuscript Accepted: February 15, 2007
Published: March 5, 2007

Virtual Issues
Vol. 2, Iss. 4 Virtual Journal for Biomedical Optics

Citation
Kenji Taira, Takeshi Hashimoto, and Hiroyuki Yokoyama, "Two-photon fluorescence imaging with a pulse source based on a 980-nm gain-switched laser diode," Opt. Express 15, 2454-2458 (2007)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-15-5-2454


Sort:  Author  |  Year  |  Journal  |  Reset  

References

  1. W. Denk, J. H. Strickler, and W. W. Webb, "Two-photon laser scanning fluorescence microscopy," Science 248, 73-76 (1990). [CrossRef] [PubMed]
  2. W. R. Zipfel, R. M. Williams, and W. W. Webb, "Nonlinear magic: multiphoton microscopy in the biosciences," Nat. Biotechnol. 21, 1369-1377 (2003). [CrossRef] [PubMed]
  3. J. Squier and M. Müller, "High resolution nonlinear microscopy: A review of sources and methods for achieving optimal imaging," Rev. Sci. Inst. 72, 2855-2867 (2001). [CrossRef]
  4. J. M. Girkin and G. McConnell, "Advances in laser sources for confocal and multiphoton microscopy," Microsc. Res. Tech. 67, 8-14 (2005). [CrossRef] [PubMed]
  5. H. Yokoyama, H. Guo, T. Yoda, K. Takashima, K. Sato, H. Taniguchi, and H. Ito, "Two-photon bioimaging with picosecond optical pulses from a semiconductor laser," Opt. Express 14, 3467-3471 (2006), http://www.opticsinfobase.org/abstract.cfm?URI=oe-14-8-3467. [CrossRef] [PubMed]
  6. A. Takada, T. Sugie, and M. Saruwatari, "High-speed picosecond optical pulse compression from gain-switched 1.3-μm distributed feedback-laser diode (DFB-LD) through highly dispersive single-mode fiber," J. Lightwave Technol. 5, 1525-1533 (1987). [CrossRef]
  7. H. Guo, K. Sato, K. Takashima, and H. Yokoyama, "Two-photon bio-imaging with a mode-locked semiconductor laser," 15th International Conference on Ultrafast Phenomena, California, USA, TuE8, July 30 - August 4 (2006).

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

Figures

Fig. 1. Fig. 2. Fig. 3.
 
Fig. 4.
 

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited