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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 15, Iss. 5 — Mar. 5, 2007
  • pp: 2490–2498

Single-organelle tracking by two-photon conversion

Wataru Watanabe, Tomoko Shimada, Sachihiro Matsunaga, Daisuke Kurihara, Kiichi Fukui, Shin-ichi Arimura, Nobuhiro Tsutsumi, Keisuke Isobe, and Kazuyoshi Itoh  »View Author Affiliations


Optics Express, Vol. 15, Issue 5, pp. 2490-2498 (2007)
http://dx.doi.org/10.1364/OE.15.002490


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Abstract

Spatial and temporal information about intracellular objects and their dynamics within a living cell are essential for dynamic analysis of such objects in cell biology. A specific intracellular object can be discriminated by photoactivatable fluorescent proteins that exhibit pronounced light-induced spectral changes. Here, we report on selective labeling and tracking of a single organelle by using two-photon conversion of a photoconvertible fluorescent protein with near-infrared femtosecond laser pulses. We performed selective labeling of a single mitochondrion in a living tobacco BY-2 cell using two-photon photoconversion of Kaede. Using this technique, we demonstrated that, in plants, the directed movement of individual mitochondria along the cytoskeletons was mediated by actin filaments, whereas microtubules were not required for the movement of mitochondria. This single-organelle labeling technique enabled us to track the dynamics of a single organelle, revealing the mechanisms involved in organelle dynamics. The technique has potential application in direct tracking of selective cellular and intracellular structures.

© 2007 Optical Society of America

OCIS Codes
(170.1790) Medical optics and biotechnology : Confocal microscopy
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(180.1790) Microscopy : Confocal microscopy
(180.2520) Microscopy : Fluorescence microscopy
(180.6900) Microscopy : Three-dimensional microscopy
(190.7110) Nonlinear optics : Ultrafast nonlinear optics

ToC Category:
Microscopy

History
Original Manuscript: December 15, 2006
Revised Manuscript: February 1, 2007
Manuscript Accepted: February 2, 2007
Published: March 5, 2007

Virtual Issues
Vol. 2, Iss. 4 Virtual Journal for Biomedical Optics

Citation
Wataru Watanabe, Tomoko Shimada, Sachihiro Matsunaga, Daisuke Kurihara, Kiichi Fukui, Shin-ichi Shin-ichi Arimura, Nobuhiro Tsutsumi, Keisuke Isobe, and Kazuyoshi Itoh, "Single-organelle tracking by two-photon conversion," Opt. Express 15, 2490-2498 (2007)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-15-5-2490


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