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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 15, Iss. 5 — Mar. 5, 2007
  • pp: 2598–2606

Metal-enhanced fluorescence of an organic fluorophore using gold particles

Jian Zhang and Joseph R. Lakowicz  »View Author Affiliations

Optics Express, Vol. 15, Issue 5, pp. 2598-2606 (2007)

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Particulate gold films were deposited on glass substrates by vapor deposition. Rabbit immunoglobulin G (IgG) was immobilized by physiosorption and then Alexa Fluor anti Rabbit IgG was bound to the protein-coated surfaces. Fluorescence was enhanced with increasing the Au thickness and reached saturation at 30 nm when Alexa Fluor555 anti IgG was used. We also examined the effect of silica spacers between the gold film and the labeled protein. The maximum enhancement was dependent on the thickness of silica and reach maximum at 10 nm. The maximum increase in intensity was about 6-fold. We also bound Alexa Fluor-680 anti IgG to the protein-coated surface, and the maximum enhancement was about 10-fold.

© 2007 Optical Society of America

OCIS Codes
(010.1080) Atmospheric and oceanic optics : Active or adaptive optics
(240.6680) Optics at surfaces : Surface plasmons

ToC Category:
Optics at Surfaces

Original Manuscript: September 5, 2006
Revised Manuscript: October 20, 2006
Manuscript Accepted: October 23, 2006
Published: March 5, 2007

Virtual Issues
Vol. 2, Iss. 4 Virtual Journal for Biomedical Optics

Jian Zhang and Joseph R. Lakowicz, "Metal-enhanced fluorescence of an organic fluorophore using gold particles," Opt. Express 15, 2598-2606 (2007)

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