Optical forced oscillation for the study of lectin-glycoprotein interaction at the cellular membrane of a Chinese hamster ovary cell

doi:10.1364/OE.15.002713

Optical forced oscillation for the study of lectin-glycoprotein interaction at the cellular membrane of a Chinese hamster ovary cell

Shang-Ling Liu, Artashes Karmenyan, Ming-Tzo Wei, Chun-Chieh Huang, Chi-Hung Lin, and Arthur Chiou »View Author Affiliations

Optics Express, Vol. 15, Issue 5, pp. 2713-2723 (2007)
http://dx.doi.org/10.1364/OE.15.002713

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Abstract

We report the application of a set of twin optical tweezers to trap and oscillate a ConA (lectin)- coated polystyrene particle and to measure its interaction with glycoprotein receptors at the cellular plasma membrane of a Chinese hamster ovary (CHO) cell. The particle was trapped between two quadratic potential wells defined by a set of twin optical tweezers and was forced to oscillate by chopping on and off one of the trapping beams. We tracked the oscillatory motion of the particle via a quadrant photodiode and measured with a lock-in amplifier the amplitude of the oscillation as a function of frequency at the fundamental component of the driving frequency over a frequency range from 10Hz to 600Hz. By analyzing the amplitude as a function of frequency for a free particle suspended in buffer solution without the presence of the CHO cell and compared with the corresponding data when the particle was interacting with the CHO cell, we deduced the transverse force constant associated with the optical trap and that associated with the interaction by treating both the optical trap and the interaction as linear springs. The force constants were determined to be approximately 2.15pN/μm for the trap and 2.53pN/μm for the lectin-glycoprotein interaction. When the CHO cell was treated with lantrunculin A, a drug that is known to destroy the cytoskeleton of the cell, the oscillation amplitude increased with time, indicating the softening of the cellular membrane, until a steady state with a smaller force constant was reached. The steady state value of the force constant depended on the drug concentration.

OCIS Codes
(140.7010) Lasers and laser optics : Laser trapping
(170.1420) Medical optics and biotechnology : Biology
(170.4520) Medical optics and biotechnology : Optical confinement and manipulation

ToC Category:
Trapping

History
Original Manuscript: January 11, 2007
Revised Manuscript: February 17, 2007
Manuscript Accepted: February 17, 2007
Published: March 5, 2007

Virtual Issues
Vol. 2, Iss. 4 Virtual Journal for Biomedical Optics

Citation
Shang-Ling Liu, Artashes Karmenyan, Ming-Tzo Wei, Chun-Chieh Huang, Chi-Hung Lin, and Arthur Chiou, "Optical forced oscillation for the study of lectin-glycoprotein interaction at the cellular membrane of a Chinese hamster ovary cell," Opt. Express 15, 2713-2723 (2007)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-15-5-2713

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References

M. M. Brandao, A. Fontes, M. L. Barjas-Castro, L. C. Barbosa, F. F. Costa, C. L. Cesar, and S. T. O. Saad, "Optical tweezers for measuring red blood cell elasticity: application to the study of drug response in sickle cell disease," Eur. J Haematol. 70, 207-211 (2003). [CrossRef] [PubMed]
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D. Leckband, "Measuring the forces that control protein interactions," Annu. Rev. Biophys. Biomol. Struct. 29, 1-26 (2000). [CrossRef] [PubMed]
G. Jiang, G. Giannone, D. R. Critchley, E. Fukumoto, and M. P. Sheetz, "Two-piconewton slip bond between fibronectin and the cytoskeleton depends on talin," Nature 424, 334-337 (2003). [CrossRef] [PubMed]
F. Qian, S. Ermilov, D. Murdock, W. E. Brownell, and B. Anvaria, "Combining optical tweezers and patch clamp for studies of cell membrane electromechanics," Rev. Sci. Instrum. 75, 2937-2942 (2004). [CrossRef] [PubMed]
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Y. Sako and A. Kusumi, "Barriers for lateral diffusion of transferrin receptors in the plasma membrane as characterized by receptor dragging by laser tweezers: fence versus tether," J. Cell Biol. 129, 1559-1574 (1995). [CrossRef] [PubMed]
J.-Y. Shao and J. Xu, "A modified micropipette aspiration technique and its application to tether formation from human neutrophils," J. Biomech. Eng. 124, 388-396 (2002). [CrossRef]
A. Ashkin and J. M. Dziedzic, "Optical trapping and manipulation of single cell using infrared laser beams," Nature 330, 769-771 (1987). [CrossRef] [PubMed]
M. T. Valentine, L. E. Dewalt, and H. D. Ou-Yang, "Forces on a colloidal particle in a polymer solution: a study using optical tweezers," J. Phys.: Condens. Matter 8, 9477-9482 (1996). [CrossRef]
L.A. Hough and H. D. Ou-Yang, "Correlated motions of two hydrodynamically coupled particles confined in separate quadratic potential wells," Phy. Rev. E, 65, 021906 (2002). [CrossRef]
L.A. Hough and H. D. Ou-Yang, "Viscoelasticity of aqueous telechelic poly(ethylene oxide) solutions: Relaxation and structure," Phy. Rev. E, 73, 031802 (2006). [CrossRef]
T. Roopa, N. Kumar, S. Bhattacharya, and G. V. Shivashankar, "Dynamics of membrane nanotubulation and DNA self-assembly," Biophys. J. 87, 974-979 (2004). [CrossRef]

Ananthakrishnan, R.
Anvaria, B.
Ashkin, A.
Bao, G.
Barbosa, L. C.
Barjas-Castro, M. L.
Bassereau, P.
Bhattacharya, S.
Bilby, C.
Brandao, M. M.
Brownell, W. E.
Cesar, C. L.
Costa, F. F.
Critchley, D. R.
Cuvelier, D.
Derenyi, I.
Dewalt, L. E.
Dziedzic, J. M.
Ebert, S.
Erickson, H. M.
Ermilov, S.
Fontes, A.
Fukumoto, E.
Giannone, G.
Guck, J.
Hough, L.A.
Jiang, G.
Kas, J.
Khan, S.
Kumar, N.
Kusumi, A.
Leckband, D.
Lenz, D.
Lincoln, B.
Mitchell, D.
Murdock, D.
Nassoy, P.
Ou-Yang, H. D.
Qian, F.
Romeyke, M.
Roopa, T.
Saad, S. T. O.
Sako, Y.
Schinkinger, S.
Shao, J.-Y.
Sheetz, M. P.
Shivashankar, G. V.
Suresh, S.
Ulvick, S.
Valentine, M. T.
Wottawah, F.
Xu, J.

Annu. Rev. Biophys. Biomol. Struct.

D. Leckband, "Measuring the forces that control protein interactions," Annu. Rev. Biophys. Biomol. Struct. 29, 1-26 (2000). [CrossRef] [PubMed]

Annual Review of Biochemistry

S. Khan and M. P. Sheetz, "Force effects on biochemical kinetics," Annual Review of Biochemistry 66, 785-805 (1997). [CrossRef] [PubMed]

Biophys. J.

D. Cuvelier, I. Derenyi, P. Bassereau, and P. Nassoy, "Coalescence of Membrane Tethers: Experiments, Theory, and Applications," Biophys. J. 88, 2714-2726 (2005). [CrossRef] [PubMed]
J. Guck, S. Schinkinger, B. Lincoln, F. Wottawah, S. Ebert, M. Romeyke, D. Lenz, H. M. Erickson, R. Ananthakrishnan, D. Mitchell, J. Kas, S. Ulvick, and C. Bilby, "Optical deformability as an inherent cell marker for testing malignant transformation and metastatic competence," Biophys. J. 88, 3689-3698 (2005). [CrossRef] [PubMed]
T. Roopa, N. Kumar, S. Bhattacharya, and G. V. Shivashankar, "Dynamics of membrane nanotubulation and DNA self-assembly," Biophys. J. 87, 974-979 (2004). [CrossRef]

Eur. J Haematol.

M. M. Brandao, A. Fontes, M. L. Barjas-Castro, L. C. Barbosa, F. F. Costa, C. L. Cesar, and S. T. O. Saad, "Optical tweezers for measuring red blood cell elasticity: application to the study of drug response in sickle cell disease," Eur. J Haematol. 70, 207-211 (2003). [CrossRef] [PubMed]

J. Biomech. Eng.

J.-Y. Shao and J. Xu, "A modified micropipette aspiration technique and its application to tether formation from human neutrophils," J. Biomech. Eng. 124, 388-396 (2002). [CrossRef]

J. Cell Biol.

Y. Sako and A. Kusumi, "Barriers for lateral diffusion of transferrin receptors in the plasma membrane as characterized by receptor dragging by laser tweezers: fence versus tether," J. Cell Biol. 129, 1559-1574 (1995). [CrossRef] [PubMed]

J. Phys.: Condens. Matter

M. T. Valentine, L. E. Dewalt, and H. D. Ou-Yang, "Forces on a colloidal particle in a polymer solution: a study using optical tweezers," J. Phys.: Condens. Matter 8, 9477-9482 (1996). [CrossRef]

Nature

A. Ashkin and J. M. Dziedzic, "Optical trapping and manipulation of single cell using infrared laser beams," Nature 330, 769-771 (1987). [CrossRef] [PubMed]
G. Jiang, G. Giannone, D. R. Critchley, E. Fukumoto, and M. P. Sheetz, "Two-piconewton slip bond between fibronectin and the cytoskeleton depends on talin," Nature 424, 334-337 (2003). [CrossRef] [PubMed]

Nature Materials

G. Bao and S. Suresh, "Cell and molecular mechanics of biological materials," Nature Materials 2, 715-725 (2003). [CrossRef] [PubMed]

Phy. Rev. E

L.A. Hough and H. D. Ou-Yang, "Correlated motions of two hydrodynamically coupled particles confined in separate quadratic potential wells," Phy. Rev. E, 65, 021906 (2002). [CrossRef]
L.A. Hough and H. D. Ou-Yang, "Viscoelasticity of aqueous telechelic poly(ethylene oxide) solutions: Relaxation and structure," Phy. Rev. E, 73, 031802 (2006). [CrossRef]

Rev. Sci. Instrum.

F. Qian, S. Ermilov, D. Murdock, W. E. Brownell, and B. Anvaria, "Combining optical tweezers and patch clamp for studies of cell membrane electromechanics," Rev. Sci. Instrum. 75, 2937-2942 (2004). [CrossRef] [PubMed]

2006, Hough, Phy. Rev. E
2005, Guck, Biophys. J.
2005, Cuvelier, Biophys. J.
2004, Qian, Rev. Sci. Instrum.
2004, Roopa, Biophys. J.
2003, Jiang, Nature
2003, Bao, Nature Materials
2003, Brandao, Eur. J Haematol.
2002, Shao, J. Biomech. Eng.
2002, Hough, Phy. Rev. E
2000, Leckband, Annu. Rev. Biophys. Biomol. Struct.
1997, Khan, Annual Review of Biochemistry
1996, Valentine, J. Phys.: Condens. Matter
1995, Sako, J. Cell Biol.
1987, Ashkin, Nature

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