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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 15, Iss. 5 — Mar. 5, 2007
  • pp: 2724–2731

Sample concentration using optical chromatography

Sean J. Hart, Alex Terray, Jonathan Arnold, and Tomasz A. Leski  »View Author Affiliations

Optics Express, Vol. 15, Issue 5, pp. 2724-2731 (2007)

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Optical chromatography is a technique for the separation of particles that capitalizes on the balance between optic and fluidic forces. When microscopic particles in a fluid flow encounter a laser beam propagating in the opposite direction, they are trapped axially along the beam. They are then optically pushed upstream from the laser focal point to rest at a point where the optic and fluidic forces on the particle balance. Because optical and fluid forces are sensitive to differences in the physical and chemical properties of a particle, both coarse and fine separations are possible. We describe how an optical chromatography beam directed into a tailored flow environment, has been adapted to operate as an optical filter for the concentration/bioenrichment of colloidal and biological samples. In this work, the demonstrated ability to concentrate spores of the biowarfare agent, Bacillus anthracis, may have significant impact in the biodefense arena. Application of these techniques and further design of fluidic and optical environments will allow for more specific identification, concentration and separation of many more microscopic particle and biological suspensions.

© 2007 Optical Society of America

OCIS Codes
(140.7010) Lasers and laser optics : Laser trapping
(170.1420) Medical optics and biotechnology : Biology

ToC Category:

Original Manuscript: December 20, 2006
Revised Manuscript: February 14, 2007
Manuscript Accepted: February 15, 2007
Published: March 5, 2007

Virtual Issues
Vol. 2, Iss. 4 Virtual Journal for Biomedical Optics

Sean J. Hart, Alex Terray, Jonathan Arnold, and Tomasz A. Leski, "Sample concentration using optical chromatography," Opt. Express 15, 2724-2731 (2007)

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