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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 15, Iss. 6 — Mar. 19, 2007
  • pp: 3047–3052

Quantitative phase microscopy with asynchronous digital holography

Kevin J. Chalut, William J. Brown, and Adam Wax  »View Author Affiliations


Optics Express, Vol. 15, Issue 6, pp. 3047-3052 (2007)
http://dx.doi.org/10.1364/OE.15.003047


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Abstract

We demonstrate a new method of measuring quantitative phase in imaging of biological materials. This method, asynchronous digital holography, employs knowledge of a moving fringe created by acousto-optic modulators to execute phase-shifting interferometry using two near-simultaneous interferograms. The method can be used to obtain quantitative phase images of dynamic biological samples on millisecond time scales. We present results on a standard sample, and on live cell samples.

© 2007 Optical Society of America

OCIS Codes
(090.2880) Holography : Holographic interferometry
(110.1650) Imaging systems : Coherence imaging

ToC Category:
Holography

History
Original Manuscript: January 16, 2007
Revised Manuscript: March 9, 2007
Manuscript Accepted: March 10, 2007
Published: March 19, 2007

Virtual Issues
Vol. 2, Iss. 4 Virtual Journal for Biomedical Optics

Citation
Kevin J. Chalut, William J. Brown, and Adam Wax, "Quantitative phase microscopy with asynchronous digital holography," Opt. Express 15, 3047-3052 (2007)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-15-6-3047


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References

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