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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 15, Iss. 6 — Mar. 19, 2007
  • pp: 3372–3383

Optics InfoBase > Optics Express > Volume 15 > Issue 6 > Page 3372

Orientational effects in the excitation and de-excitation of single molecules interacting with donut-mode laser beams

Peter Dedecker, Benoît Muls, Johan Hofkens, Jörg Enderlein, and Jun-ichi Hotta  »View Author Affiliations


Optics Express, Vol. 15, Issue 6, pp. 3372-3383 (2007)
http://dx.doi.org/10.1364/OE.15.003372


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Abstract

The interactions between single molecules and three-dimensional donut modes in fluorescence microscopy are discussed based on the vector diffraction theory of light. We find that the use of donut modes generated from a linearly polarized laser beam can yield information about the orientation of immobilized single molecules, allowing for their use in orientational imaging. While fairly insensitive over a range of orientations, this technique is seen to be very sensitive for the subset of orientations where the transition dipole of the molecule is oriented close to the optical axis of the microscope and perpendicular to the input polarization. In a second part of the paper we discuss the impact of the molecular orientation on the resolution improvement in STED microscopy. We find that, even for circularly polarized excitation light, the expected resolution improvement depends on the orientation of the molecule relative to the optical axis of the microscope.

© 2007 Optical Society of America

OCIS Codes
(100.6640) Image processing : Superresolution
(140.3300) Lasers and laser optics : Laser beam shaping
(180.2520) Microscopy : Fluorescence microscopy

ToC Category:
Microscopy

History
Original Manuscript: January 16, 2007
Revised Manuscript: February 28, 2007
Manuscript Accepted: February 28, 2007
Published: March 19, 2007

Virtual Issues
Vol. 2, Iss. 4 Virtual Journal for Biomedical Optics

Citation
Peter Dedecker, Benoît Muls, Johan Hofkens, Jörg Enderlein, and Jun-ichi Hotta, "Orientational effects in the excitation and de-excitation of single molecules interacting with donut-mode laser beams," Opt. Express 15, 3372-3383 (2007)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-15-6-3372


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