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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 15, Iss. 7 — Apr. 2, 2007
  • pp: 3998–4007

Imaging 2D optical diffuse reflectance in skeletal muscle

Janaka Ranasinghesagara and Gang Yao  »View Author Affiliations

Optics Express, Vol. 15, Issue 7, pp. 3998-4007 (2007)

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We discovered a unique pattern of optical reflectance from fresh prerigor skeletal muscles, which can not be described using existing theories. A numerical fitting function was developed to quantify the equi-intensity contours of acquired reflectance images. Using this model, we studied the changes of reflectance profile during stretching and rigor process. We found that the prominent anisotropic features diminished after rigor completion. These results suggested that muscle sarcomere structures played important roles in modulating light propagation in whole muscle. When incorporating the sarcomere diffraction in a Monte Carlo model, we showed that the resulting reflectance profiles quantitatively resembled the experimental observation.

© 2007 Optical Society of America

OCIS Codes
(170.3660) Medical optics and biotechnology : Light propagation in tissues
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.5280) Medical optics and biotechnology : Photon migration

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: January 16, 2007
Revised Manuscript: March 21, 2007
Manuscript Accepted: March 23, 2007
Published: April 2, 2007

Virtual Issues
Vol. 2, Iss. 5 Virtual Journal for Biomedical Optics

Janaka Ranasinghesagara and Gang Yao, "Imaging 2D optical diffuse reflectance in skeletal muscle," Opt. Express 15, 3998-4007 (2007)

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