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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 15, Iss. 7 — Apr. 2, 2007
  • pp: 4008–4017

Fibered confocal spectroscopy and multicolor imaging system for in vivo fluorescence analysis

Florence Jean, Genevieve Bourg-Heckly, and Bertrand Viellerobe  »View Author Affiliations

Optics Express, Vol. 15, Issue 7, pp. 4008-4017 (2007)

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We report the design and implementation of spectroscopic and multicolor imaging capabilities into a fibered confocal fluorescence microscope (FCFM) already capable of in vivo imaging. The real time imaging device and the high resolution fiber probe make this system the first reported capable of performing multi color detection in the field of FCFM. The advantages of the system will allow in vivo morphological and functional imaging. Preliminary experiments were carried out in tissue samples to demonstrate the potential of the technique. The quality of the axial sectioning achieved in the confocal fluorescence spectroscopy mode is demonstrated experimentally, and applications to multicolor imaging are shown.

© 2007 Optical Society of America

OCIS Codes
(110.2350) Imaging systems : Fiber optics imaging
(170.1790) Medical optics and biotechnology : Confocal microscopy
(170.2150) Medical optics and biotechnology : Endoscopic imaging
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(170.5810) Medical optics and biotechnology : Scanning microscopy
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: December 21, 2006
Revised Manuscript: March 26, 2007
Manuscript Accepted: March 26, 2007
Published: April 2, 2007

Virtual Issues
Vol. 2, Iss. 5 Virtual Journal for Biomedical Optics

Florence Jean, Genevieve Bourg-Heckly, and Bertrand Viellerobe, "Fibered confocal spectroscopy and multicolor imaging system for in vivo fluorescence analysis," Opt. Express 15, 4008-4017 (2007)

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