OSA's Digital Library

Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 15, Iss. 8 — Apr. 16, 2007
  • pp: 4634–4646

Investigation of the point spread function of surface plasmon-coupled emission microscopy

Wai Teng Tang, Euiheon Chung, Yang-Hyo Kim, Peter T. C. So, and Colin J. R. Sheppard  »View Author Affiliations


Optics Express, Vol. 15, Issue 8, pp. 4634-4646 (2007)
http://dx.doi.org/10.1364/OE.15.004634


View Full Text Article

Enhanced HTML    Acrobat PDF (408 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

Surface plasmon-coupled emission microscopy (SPCEM) was proposed as a high sensitivity technique that makes use of a thin layer of metal deposited on glass slides to efficiently excite fluorophores and to collect the emission light. However, the theoretical aspect of SPCEM imaging has not been well studied. We propose a model for SPCEM and show, through theoretical analysis and empirical results, that the point spread function of SPCEM is irregular and has an annular-like structure, significantly different from the familiar point spread function of the conventional wide-field microscopy. This result is due to the highly polarized and anisotropic emission caused by the metal layer.

© 2007 Optical Society of America

OCIS Codes
(180.2520) Microscopy : Fluorescence microscopy
(240.6680) Optics at surfaces : Surface plasmons
(260.2110) Physical optics : Electromagnetic optics

ToC Category:
Microscopy

History
Original Manuscript: February 15, 2007
Revised Manuscript: March 27, 2007
Manuscript Accepted: March 27, 2007
Published: April 3, 2007

Virtual Issues
Vol. 2, Iss. 5 Virtual Journal for Biomedical Optics

Citation
Wai Teng Tang, Euiheon Chung, Yang-Hyo Kim, Peter T. C. So, and Colin J. R. Sheppard, "Investigation of the point spread function of surface plasmon-coupled emission microscopy," Opt. Express 15, 4634-4646 (2007)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-15-8-4634


Sort:  Author  |  Year  |  Journal  |  Reset  

References

  1. D. Axelrod, "Total internal reflection microscopy in cell biology," Traffic 2, 764-774 (2001). [CrossRef] [PubMed]
  2. S. E. Sund, and D. Axelrod, "Actin dynamics at the living cell submembrane imaged by total internal reflection fluorescence photobleaching," Biophys. J. 79, 1655-1669 (2000). [CrossRef] [PubMed]
  3. J. A. Steyer, H. Horstmann, and W. Almers, "Transport, docking and exocytosis of single secretory granules in live chromaffin cells," Nature 388, 474-478 (1997). [CrossRef] [PubMed]
  4. E. L. Schmid, A. Tairi, R. Hovius, and H. Vogel, "Screening ligands for membrane protein receptors by total internal reflection fluorescence: The 5-HT3 serotonin receptor," Anal. Chem. 70, 1331-1338 (1998). [CrossRef] [PubMed]
  5. J. Borejdo, Z. Gryzyncski, N. Calander, P. Muthu, and I. Gryzyncski, "Application of surface plasmon coupled emission to study of muscle," Biophys. J. 91, 2626-2635 (2006). [CrossRef] [PubMed]
  6. J. R. Lakowicz, "Directional surface plasmon-coupled emission: a new method for high sensitivity detection," Biochem. and Biophys. Res. Comm. 307, 435-439 (2003). [CrossRef]
  7. J. R. Lakowicz, "Radiative decay engineering 3. surface plasmon-coupled directional emission," Anal. Biochem. 324, 153-169 (2004). [CrossRef]
  8. I. Gryzyncski, J. Malicka, Z. Gryzyncski, and J. R. Lakowicz, "Radiative decay engineering 4. experimental studies of surface-plasmon coupled directional emission," Anal. Biochem. 324, 170-1822004. [CrossRef]
  9. J. Malicka, I. Gryzyncski, Z. Gryzyncski, and J. R. Lakowicz, "DNA hybridization using surface plasmoncoupled emssion," Anal. Chem. 75, 6629-6633 (2003). [CrossRef] [PubMed]
  10. J. Borejdo, N. Calander, Z. Gryzyncski, and I. Gryzyncski, "Fluorescence correlation spectroscopy in surface plasmon coupled emission microscope," Opt. Express 14, 7878-7888 (2006). [CrossRef] [PubMed]
  11. H. Raether, Surface Plasmons on Smooth and Rough Surfaces and on Gratings, (Springer-Verlag, 1986)
  12. B. Richards and E. Wolf, "Electromagnetic diffraction in optical systems. II. structure of the image field in an aplanatic system," Proc. Roy. Soc. (London) A 253, 358-379 (1959). [CrossRef]
  13. E. Wolf, "Electromagnetic diffraction in optical systems. I. an integral representation of the image field. Proc. Roy. Soc. (London) A 253, 349-357 (1959). [CrossRef]
  14. P. Torok, "Propagation of electromagnetic dipole waves through dielectric interfaces," Opt. Lett. 25, 1463-1465 (2000). [CrossRef]
  15. H. F. Arnoldus and J. T. Foley. "Transmission of dipole radiation through interfaces and the phenomenon of anti-critical angles," J. Opt. Soc. Am. A 21, 1109-1117 (2004). [CrossRef]
  16. E. H. Hellen and D. Axelrod, "Fluorescence emission at dielectric and metal-film interfaces," J. Opt. Soc. Am. B 4, 337-349 (1987). [CrossRef]
  17. P. Torok, P. Varga, Z. Laczik, and G. R. Booker. "Electromagnetic diffraction of light focused through a planar interface between materials of mismatched refractive indices: an integral representation," J. Opt. Soc. Am. A 12, 325-332 (1995). [CrossRef]
  18. P. Torok, P. D. Higdon, and T. Wilson, "Theory for confocal and conventional microscopes imaging small dielectric scatterers," J. Mod. Opt. 45, 1681-1698 (1998). [CrossRef]
  19. P. Torok and C. J. R. Sheppard, "The role of pinhole size in high-aperture two- and three-photon microscopy," in Confocal and Two-Photon Microscopy: Foundations, Applications, and Advances, Alberto Diaspro, ed. (Wiley- Liss, Inc., New York, 2002), pp. 127-151.
  20. J. Enderlein, and M . Böhmer, "Influence of interface-dipole interactions on the efficiency of fluorescence light collection near surfaces," Opt. Lett. 28, 941-943 (2003). [CrossRef] [PubMed]
  21. C. J. R. Sheppard and P. Torok, "An electromagnetic theory of imaging in fluorescence microscopy, and imaging in polarization fluorescence microscopy," Bioimaging 5, 205-218 (1997). [CrossRef]
  22. E. D. Palik, Handbook of Optical Constants of Solids, (Academic Press, 1985).
  23. E. Chung, D. Kim, and P. T. So, "Extended resolution wide-field optical imaging: objective-launched standingwave total internal reflection fluorescence microscopy," Opt. Lett. 31, 945-947 (2006). [CrossRef] [PubMed]
  24. M. A. A. Neil, R. Juskaitis, and T. Wilson, "Method of obtaining optical sectioning by using structured light in a conventional microscope," Opt. Lett. 22, 1905-1907 (1997). [CrossRef]
  25. J. Enderlein, and T. Ruckstuhl, "The efficiency of surface-plasmon coupled emission for sensitive fluorescence detection," Opt. Express 13, 8855-8865 (2005). [CrossRef] [PubMed]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.


« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited