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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 15, Iss. 8 — Apr. 16, 2007
  • pp: 4634–4646

Investigation of the point spread function of surface plasmon-coupled emission microscopy

Wai Teng Tang, Euiheon Chung, Yang-Hyo Kim, Peter T. C. So, and Colin J. R. Sheppard  »View Author Affiliations

Optics Express, Vol. 15, Issue 8, pp. 4634-4646 (2007)

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Surface plasmon-coupled emission microscopy (SPCEM) was proposed as a high sensitivity technique that makes use of a thin layer of metal deposited on glass slides to efficiently excite fluorophores and to collect the emission light. However, the theoretical aspect of SPCEM imaging has not been well studied. We propose a model for SPCEM and show, through theoretical analysis and empirical results, that the point spread function of SPCEM is irregular and has an annular-like structure, significantly different from the familiar point spread function of the conventional wide-field microscopy. This result is due to the highly polarized and anisotropic emission caused by the metal layer.

© 2007 Optical Society of America

OCIS Codes
(180.2520) Microscopy : Fluorescence microscopy
(240.6680) Optics at surfaces : Surface plasmons
(260.2110) Physical optics : Electromagnetic optics

ToC Category:

Original Manuscript: February 15, 2007
Revised Manuscript: March 27, 2007
Manuscript Accepted: March 27, 2007
Published: April 3, 2007

Virtual Issues
Vol. 2, Iss. 5 Virtual Journal for Biomedical Optics

Wai Teng Tang, Euiheon Chung, Yang-Hyo Kim, Peter T. C. So, and Colin J. R. Sheppard, "Investigation of the point spread function of surface plasmon-coupled emission microscopy," Opt. Express 15, 4634-4646 (2007)

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