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Optics Express

Optics Express

  • Editor: C. Martijin de Sterke
  • Vol. 15, Iss. 9 — Apr. 30, 2007
  • pp: 5366–5375

Dynamic disorder in horseradish peroxidase observed with total internal reflection fluorescence correlation spectroscopy

Kai Hassler, Per Rigler, Hans Blom, Rudolf Rigler, Jerker Widengren, and Theo Lasser  »View Author Affiliations


Optics Express, Vol. 15, Issue 9, pp. 5366-5375 (2007)
http://dx.doi.org/10.1364/OE.15.005366


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Abstract

This paper discusses the application of objective-type total internal reflection fluorescence correlation spectroscopy (TIR-FCS) to the study of the kinetics of immobilized horseradish peroxidase on a single molecule level. Objective-type TIR-FCS combines the advantages of FCS with TIRF microscopy in a way that allows for simultaneous ultra-sensitive spectroscopic measurements using a single-point detector and convenient localization of single molecules on a surface by means of parallel imaging.

© 2007 Optical Society of America

OCIS Codes
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(300.2530) Spectroscopy : Fluorescence, laser-induced
(300.6490) Spectroscopy : Spectroscopy, surface

ToC Category:
Spectroscopy

History
Original Manuscript: March 9, 2007
Revised Manuscript: April 16, 2007
Manuscript Accepted: April 16, 2007
Published: April 18, 2007

Virtual Issues
Vol. 2, Iss. 5 Virtual Journal for Biomedical Optics

Citation
Kai Hassler, Per Rigler, Hans Blom, Rudolf Rigler, Jerker Widengren, and Theo Lasser, "Dynamic disorder in horseradish peroxidase observed with total internal reflection fluorescence correlation spectroscopy," Opt. Express 15, 5366-5375 (2007)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-15-9-5366


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