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Optics Express

Optics Express

  • Editor: C. Martijin de Sterke
  • Vol. 15, Iss. 9 — Apr. 30, 2007
  • pp: 5589–5594

Acoustical nanometre-scale vibrations of live cells detected by a near-field optical setup

Rosaria Piga, Ruggero Micheletto, and Yoichi Kawakami  »View Author Affiliations

Optics Express, Vol. 15, Issue 9, pp. 5589-5594 (2007)

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The Scanning Near-field Optical Microscope (SNOM) is able to detect tiny vertical movement on the cell membrane in the range of only 1 nanometer or less, about 3 orders of magnitude better than conventional optical microscopes. Here we show intriguing data of cell membrane nanometer-scale dynamics associated to different phenomena of the cell’s life, such as cell cycle and cell death, on rat pheochromocytoma line PC12. Working in culture medium with alive and unperturbed samples, we could detect nanometer-sized movements; Fourier components revealed a clear distinct behavior associated to regulation of neurite outgrowth and changes on morphology after necrotic stimulus.

© 2007 Optical Society of America

OCIS Codes
(070.1060) Fourier optics and signal processing : Acousto-optical signal processing
(170.1530) Medical optics and biotechnology : Cell analysis

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: March 5, 2007
Revised Manuscript: April 23, 2007
Manuscript Accepted: April 23, 2007
Published: April 24, 2007

Virtual Issues
Vol. 2, Iss. 5 Virtual Journal for Biomedical Optics

Rosaria Piga, Ruggero Micheletto, and Yoichi Kawakami, "Acoustical nanometre-scale vibrations of live cells detected by a near-field optical setup," Opt. Express 15, 5589-5594 (2007)

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