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Optics Express

Optics Express

  • Editor: C. Martijin de Sterke
  • Vol. 15, Iss. 9 — Apr. 30, 2007
  • pp: 5595–5603

Highly sensitive spectroscopic detection of heme-protein submonolayer films by channel integrated optical waveguide

Ismail Emre Araci, Sergio B. Mendes, Nasuhi Yurt, Seppo Honkanen, and N. Peyghambarian  »View Author Affiliations

Optics Express, Vol. 15, Issue 9, pp. 5595-5603 (2007)

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A highly sensitive technique based on optical absorption using a single-mode, channel integrated optical waveguide is described for broad spectral band detection and analysis of heme-containing protein films at a glass/water interface. Fabrication steps and device characteristics of optical waveguides suitable for operation in the wavelength range of 400 – 650 nm are described. Experimental results reported here show a limit of detection smaller than 100 pg/cm2 for a submonolayer of ferricytochrome c by probing the Soret transition band with a 406-nm semiconductor diode laser propagating in a single-mode, ion-exchanged channel waveguide. By taking advantage of the exceptionally low limit of detection, we examined the adsorption isotherm of cytochrome c on a glass surface with unprecedented detail. Unlike other surface-specific techniques (e.g. SPR, integrated optic Mach-Zehnder interferometer) that probe local refractive-index changes and therefore are very susceptible to temperature fluctuations, the integrated optical waveguide absorption technique probes molecular-specific transition bands and is expected to be less vulnerable to environmental perturbations.

© 2007 Optical Society of America

OCIS Codes
(130.2790) Integrated optics : Guided waves
(130.6010) Integrated optics : Sensors
(230.7380) Optical devices : Waveguides, channeled

ToC Category:
Integrated Optics

Original Manuscript: March 9, 2007
Revised Manuscript: April 9, 2007
Manuscript Accepted: April 17, 2007
Published: April 24, 2007

Virtual Issues
Vol. 2, Iss. 5 Virtual Journal for Biomedical Optics

Ismail E. Araci, Sergio B. Mendes, Nasuhi Yurt, Seppo Honkanen, and N. Peyghambarian, "Highly sensitive spectroscopic detection of heme-protein submonolayer films by channel integrated optical waveguide," Opt. Express 15, 5595-5603 (2007)

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