OSA's Digital Library

Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 16, Iss. 10 — May. 12, 2008
  • pp: 7102–7118

Molecular interferometric imaging

Ming Zhao, Xuefeng Wang, and David D. Nolte  »View Author Affiliations


Optics Express, Vol. 16, Issue 10, pp. 7102-7118 (2008)
http://dx.doi.org/10.1364/OE.16.007102


View Full Text Article

Enhanced HTML    Acrobat PDF (420 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

Common-path in-line shearing interferometry, combined with pixel-array imaging, provides a surface metrology that achieves 15 pm surface height resolution. An eighth-wave thermal oxide on silicon generates a reference wave locked in the condition of phase quadrature for phase-to-intensity conversion that makes surface height or index variations directly detectable by an imaging system. The scaling surface mass sensitivity for the surface metrology application is Sscal=7 fg/mm under 40× magnification with a molecular resolution of approximately 12 IgG molecules within a pixel, limited by the surface roughness of the substrate. When applied to reverse-phase immunoassays in an antibody microarray format under 7× magnification, the current limit of detection is 10 ng/ml for 1 hour incubation, limited by biological and chemical variability. The biosensor is compatible with real-time binding measurements under active flow conditions with a binding dynamic range per well of 103 and a mass sensitivity of 2 pg/mm2.

© 2008 Optical Society of America

OCIS Codes
(170.0110) Medical optics and biotechnology : Imaging systems
(170.0180) Medical optics and biotechnology : Microscopy
(170.1470) Medical optics and biotechnology : Blood or tissue constituent monitoring
(110.3175) Imaging systems : Interferometric imaging

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: September 7, 2007
Revised Manuscript: December 4, 2007
Manuscript Accepted: April 29, 2008
Published: May 2, 2008

Virtual Issues
Vol. 3, Iss. 6 Virtual Journal for Biomedical Optics

Citation
Ming Zhao, Xuefeng Wang, and David D. Nolte, "Molecular interferometric imaging," Opt. Express 16, 7102-7118 (2008)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-16-10-7102


Sort:  Author  |  Year  |  Journal  |  Reset  

References

  1. N. L. Anderson and N. G. Anderson, "The human plasma proteome - History, character, and diagnostic prospects," Mol. Cell. Proteomics 1, 845-867 (2002). [CrossRef] [PubMed]
  2. G. Gauglitz, "Direct optical sensors: principles and selected applications," Anal. Bioanal. Chem. 381, 141-155 (2005). [CrossRef] [PubMed]
  3. J. Homola, "Present and future of surface plasmon resonance biosensors," Anal.Bioanal. Chem. 377, 528-539 (2003). [CrossRef] [PubMed]
  4. B. Cunningham, P. Li, and J. Pepper, "Colorimetric resonant reflection as a direct biochemical assay technique," Sens. Actuators B 81, 316-328 (2002). [CrossRef]
  5. M. Armani, A. P. Kulkarni, S. E. Fraser, R. C. Flagen, and K. J. Vahala, "Label-free, single-molecule detection with optical microcavities," Science 317, 783-787 (2007). [CrossRef] [PubMed]
  6. M. M. Varma, D. D. Nolte, H. D. Inerowicz, and F. E. Regnier, "Spinning-disk self-referencing interferometry of antigen-antibody recognition," Opt. Lett. 29, 950-952 (2004). [CrossRef] [PubMed]
  7. D. D. Nolte and M. Zhao, "Scaling mass sensitivity of the BioCD at 0.25 pg/mm," Proc. SPIE 6380, 63800J (2006). [CrossRef]
  8. M. M. Varma, H. D. Inerowicz, F. E. Regnier, and D. D. Nolte, "High-speed label-free detection by spinning-disk micro-interferometry," Biosens. Bioelectron. 19, 1371-1376 (2004). [CrossRef] [PubMed]
  9. L. Peng, M. M. Varma, W. Cho, F. E. Regnier, and D. D. Nolte, "Adaptive interferometry of protein on a BioCD," Appl. Opt. 46, 5384-5395 (2007). [CrossRef] [PubMed]
  10. M. Zhao, W. R. Cho, F. Regnier, and D. Nolte, "Differential phase-contrast BioCD biosensor," Appl. Opt. 46, 6196-6209 (2007). [CrossRef] [PubMed]
  11. X. F. Wang, M. Zhao, and D. D. Nolte, "Common-path interferometric detection of protein monolayer on the BioCD," Appl. Opt. 46, 7836-7849 (2007). [CrossRef] [PubMed]
  12. M. Bras, V. Dugas, F. Bessueille, J. P. Cloared, J. R. Martin, M. Cabrera, J. P. Chauvet, E. Souteyrand, and M. Garrigues, "Optimization of a silicon/silicon dioxide substrate for fluorescence DNA microarray," Biosens. and Bioelectron. 20, 797-806 (2004). [CrossRef]
  13. T. J. Gao, J.H. Lu, and L. J. Rothberg, "Biomolecular sensing using near-null single wavelength arrayed imaging reflectometry," Anal. Chem. 78, 6622-6627 (2006). [CrossRef] [PubMed]
  14. K. Johansen, H. Arwin, I. Lundstrom, and B. Liedberg, "Imaging surface plasmon resonance sensor based on multiple wavelengths: Sensitivity considerations," Rev. Sci. Instrum. 71, 3530-3538 (2000). [CrossRef]
  15. J. Dyson, "Common-Path Interferometer For Testing Purposes," J. Opt. Soc. Am. 47, 386-390 (1957). [CrossRef]
  16. P. Hariharan, Optical Interferometry (Elsevier, 2003).
  17. V. Kiessling and L. K. Tamm, "Measuring distances in supported bilayers by fluorescence interference-contrast microscopy: Polymer supports and SNARE proteins," Biophys. J. 84, 408-418 (2003). [CrossRef] [PubMed]
  18. V. Jacobsen, P. Stoller, C. Brunner, V. Vogel, and V. Sandoghdar, "Interferometric optical detection and tracking of very small gold nanoparticles at a water-glass interface," Opt. Express [PubMed]
  19. O. S. Heavens, Optical Properties of Thin Solid Films (Dover, 1991).

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

Multimedia

Multimedia FilesRecommended Software
» Media 1: AVI (2873 KB)      QuickTime

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited