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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 16, Iss. 10 — May. 12, 2008
  • pp: 7224–7232

Three-dimensional in vivo imaging by a handheld dual-axes confocal microscope

Hyejun Ra, Wibool Piyawattanametha, Michael J. Mandella, Pei-Lin Hsiung, Jonathan Hardy, Thomas D. Wang, Christopher H. Contag, Gordon S. Kino, and Olav Solgaard  »View Author Affiliations


Optics Express, Vol. 16, Issue 10, pp. 7224-7232 (2008)
http://dx.doi.org/10.1364/OE.16.007224


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Abstract

We present a handheld dual-axes confocal microscope that is based on a two-dimensional microelectromechanical systems (MEMS) scanner. It performs reflectance and fluorescence imaging at 488 nm wavelength, with three-dimensional imaging capability. The fully packaged microscope has a diameter of 10 mm and acquires images at 4 Hz frame rate with a maximum field of view of 400 µm×260 µm. The transverse and axial resolutions of the handheld probe are 1.7 µm and 5.8 µm, respectively. Capability to perform real time small animal imaging is demonstrated in vivo in transgenic mice.

© 2008 Optical Society of America

OCIS Codes
(170.1790) Medical optics and biotechnology : Confocal microscopy
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(170.5810) Medical optics and biotechnology : Scanning microscopy
(170.6900) Medical optics and biotechnology : Three-dimensional microscopy

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: February 11, 2008
Revised Manuscript: April 7, 2008
Manuscript Accepted: April 14, 2008
Published: May 5, 2008

Virtual Issues
Vol. 3, Iss. 6 Virtual Journal for Biomedical Optics

Citation
Hyejun Ra, Wibool Piyawattanametha, Michael J. Mandella, Pei-Lin Hsiung, Jonathan Hardy, Thomas D. Wang, Christopher H. Contag, Gordon S. Kino, and Olav Solgaard, "Three-dimensional in vivo imaging by a handheld dual-axes confocal microscope," Opt. Express 16, 7224-7232 (2008)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-16-10-7224


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References

  1. J. Pawley, ed., Handbook of Biological Confocal Microscopy, 3rd ed. (Plenum, New York, 1996).
  2. D. L. Dickensheets and G. S. Kino, "Silicon-micromachined scanning confocal microscope," J. Microelectromech. Syst. 7, 38-47 (1998). [CrossRef]
  3. W. Piyawattanametha, H. Toshiyoshi, J. LaCosse, and M. C. Wu, "Surface-micromachined confocal scanning optical microscope," in Conference on Lasers and Electro-Optics, Technical Digest (Optical Society of America, 2000), pp. 447-448.
  4. K. Murakami, A. Murata, T. Suga, H. Kitagawa, Y. Kamiya, M. Kubo, K. Matsumoto, H. Miyajima, and M. Katashiro, "A miniature confocal optical microscope with MEMS gimbal scanner," in Technical Digest of Transducers (Boston, 2003), pp. 587-590.
  5. S. Kwon and L. P. Lee, "Micromachined transmissive scanning confocal microscope," Opt. Lett. 29, 706-708 (2004). [CrossRef] [PubMed]
  6. H.-J. Shin, M. C. Pierce, D. Lee, H. Ra, O. Solgaard, and R. Richards-Kortum, "Fiber-optic confocal microscope using a MEMS scanner and miniature objective lens," Opt. Express 15, 9113-9122 (2007), http://www.opticsinfobase.org/abstract.cfm?URI=oe-15-15-9113 [CrossRef] [PubMed]
  7. T. D. Wang, M. J. Mandella, C. H. Contag, and G. S. Kino, "Dual-axis confocal microscope for high-resolution in vivo imaging," Opt. Lett. 28, 414-416 (2003). [CrossRef] [PubMed]
  8. L. K. Wong, M. J. Mandella, G. S. Kino, and T. D. Wang, "Improved rejection of multiply scattered photons in confocal microscopy using dual-axes architecture," Opt. Lett. 32, 1674-1676 (2007). [CrossRef] [PubMed]
  9. H. Ra, W. Piyawattanametha, Y. Taguchi, D. Lee, M. J. Mandella, and O. Solgaard, "Two-Dimensional MEMS Scanner for Dual-Axes Confocal Microscopy," J. Microelectromech. Syst. 16, 969-976 (2007). [CrossRef]
  10. W. Piyawattanametha, J. T. C. Liu, M. J. Mandella, H. Ra, L. K. Wong, P. Hsiung, T. D. Wang, G. S. Kino, and O. Solgaard, "MEMS Based Dual-axes Confocal Reflectance Handheld Microscope for in vivo Imaging," in Proceedings of IEEE International Conference on Optical MicroElectroMechanical Systems, (Institute of Electrical and Electronics Engineers, Montana, August, 2006), pp. 164-165.
  11. J. T. C. Liu, M. J. Mandella, H. Ra, L. K. Wong, O. Solgaard, G. S. Kino, W. Piyawattanametha, C. H. Contag, and T. D. Wang, "Miniature near-infrared dual-axes confocal microscope utilizing a two-dimensional microelectromechanical systems scanner," Opt. Lett. 32, 256-258 (2007). [CrossRef] [PubMed]
  12. W. Piyawattanametha, H. Ra, M. J. Mandella, J. T. C. Liu, L. K. Wong, C. B. Du, T. D. Wang, C. H. Contag, G. S. Kino, and O. Solgaard, "Three-dimensional in vivo Real Time Imaging by a Miniature Dual-axes Confocal Microscope based on a Two-dimensional MEMS Scanner," in Proceedings of International Conference on Solid-State Sensors, Actuators, and Microsystems, (Lyon, France, June, 2007), pp. 439-442.
  13. H. Ra, W. Piyawattanametha, M. J. Mandella, J. T. C. Liu, L. K. Wong, T. D. Wang, C. H. Contag, G. S. Kino, and O. Solgaard, "Three-Dimensional in vivo Reflectance and Fluorescence Imaging by a Handheld Dual-Axes Confocal Microscope," in Conference on Lasers and Electro-Optics /Quantum Electronics and Laser Science and Photonic Applications Systems Technologies, Technical Digest (CD) (Optical Society of America, May, 2007), paper CTuEE1, http://www.opticsinfobase.org/abstract.cfm?URI=CLEO-2007-CTuEE1
  14. A.-K. Hadjantonakis, M. Gertsenstein, M. Ikawa, M. Okabe, and A. Nagy, "Generating green fluorescent mice by germline transmission of green fluorescent ES cells," Mech. Dev. 76, 79-90 (1998). [CrossRef] [PubMed]
  15. A. E. Siegman, M. W. Sasnett, and T. F. Johnston, Jr., "Choice of clip levels for beam width measurements using knife-edge techniques," IEEE J. Quantum Electron. 27, 1098-1104 (1991). [CrossRef]

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