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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 16, Iss. 10 — May. 12, 2008
  • pp: 7244–7250

Three-dimensional imaging in three-dimensional optical multi-beam micromanipulation

Jeppe Seidelin Dam, Ivan R. Perch-Nielsen, Darwin Palima, and Jesper Glückstad  »View Author Affiliations


Optics Express, Vol. 16, Issue 10, pp. 7244-7250 (2008)
http://dx.doi.org/10.1364/OE.16.007244


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Abstract

In this work, we present a method providing real-time, low cost, three-dimensional imaging in a three-dimensional optical micromanipulation system. The three-dimensional imaging system is based on a small form factor LED based projector. The projector is used to dynamically shape the rear illumination light in a counter-propagating beam-trapping setup. This allows us to produce stereoscopic images, from which the human brain can construct a three-dimensional image, or alternatively image analysis can be applied by a computer, thereby obtaining true three-dimensional coordinates in real-time for the trapped objects.

© 2008 Optical Society of America

OCIS Codes
(170.4520) Medical optics and biotechnology : Optical confinement and manipulation
(180.6900) Microscopy : Three-dimensional microscopy

ToC Category:
Optical Trapping and Manipulation

History
Original Manuscript: February 21, 2008
Revised Manuscript: April 14, 2008
Manuscript Accepted: April 29, 2008
Published: May 5, 2008

Virtual Issues
Vol. 3, Iss. 6 Virtual Journal for Biomedical Optics

Citation
Jeppe S. Dam, Ivan R. Perch-Nielsen, Darwin Palima, and Jesper Glückstad, "Three-dimensional imaging in three-dimensional optical multi-beam micromanipulation," Opt. Express 16, 7244-7250 (2008)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-16-10-7244


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References

  1. D. G. Grier, "A revolution in optical manipulation," Nature 424, 810-816 (2003). [CrossRef] [PubMed]
  2. A. Ashkin, "Acceleration and trapping of particles by radiation pressure," Phys. Rev. Lett. 24, 156 (1970). [CrossRef]
  3. P. J. Rodrigo, V. R. Daria, and J. Glückstad, "Real-time three-dimensional optical micromanipulation of multiple particles and living cells," Opt. Lett. 29, 2270-2272 (2004). [CrossRef] [PubMed]
  4. A. Isomura, N. Magome, M. I. Kohira, and K. Yoshikawa, "Toward the stable optical trapping of a droplet with counter laser beams under microgravity," Chem. Phys. Lett. 429, 321-325 (2006). [CrossRef]
  5. S. -H. Lee and D. G. Grier, "Holographic microscopy of holographically trapped three-dimensional structures," Opt. Express 15, 1505-1512 (2007). [CrossRef] [PubMed]
  6. I. Perch-Nielsen, P. Rodrigo, and J. Glückstad, "Real-time interactive 3D manipulation of particles viewed in two orthogonal observation planes," Opt. Express 13, 2852-2857 (2005). [CrossRef] [PubMed]
  7. P. J. Rodrigo, I. R. Perch-Nielsen, C. A. Alonzo, and J. Glückstad, "GPC-based optical micromanipulation in 3D real-time using a single spatial light modulator," Opt. Express 14, 13107-13112 (2006). [CrossRef] [PubMed]
  8. J. S. Dam, P. J. Rodrigo, I. R. Perch-Nielsen, and J. Glückstad, "Fully automated beam-alignment and single stroke guided manual alignment of counter-propagating multi-beam based optical micromanipulation systems," Opt. Express 15, 7968-7973 (2007). [CrossRef] [PubMed]
  9. S. Delica, and C. M. Blanca, "Wide-field depth-sectioning fluorescence microscopy using projector-generated patterned illumination," Appl. Opt. 46, 7237-7243 (2007). [CrossRef] [PubMed]
  10. E. C. Samson and C. M. Blanca, "Dynamic contrast enhancement in widefield microscopy using projector-generated illumination patterns," New J. Phys. 9363 (2007). [CrossRef]
  11. P. J. Rodrigo, I. R. Perch-Nielsen, and J. Glückstad, "Three-dimensional forces in GPC-based counterpropagating-beam traps," Opt. Express 14, 5812-5822 (2006). [CrossRef] [PubMed]
  12. J. S. Dam, P. J. Rodrigo, I. R. Perch-Nielsen, C. A. Alonzo, and J. Glückstad, "Computerized "drag-and-drop" alignment of GPC-based optical micromanipulation system," Opt. Express 15, 1923-1931 (2007). [CrossRef] [PubMed]
  13. K. Burton, "An aperture-shifting light-microscopic method for rapidly quantifying positions of cells in 3D matrices," Cytometry Part A 54, 125-131 (2003). [CrossRef]

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