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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 16, Iss. 11 — May. 26, 2008
  • pp: 7958–7968

Dynamics of femtosecond laser photo-modification of collagen fibers

Vladimir Hovhannisyan, Wen Lo, Chieh Hu, Shean-Jen Chen, and Chen Yuan Dong  »View Author Affiliations

Optics Express, Vol. 16, Issue 11, pp. 7958-7968 (2008)

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In this work, we investigate the non-ablative, non-thermal photo-modification of collagen fibers by femtosecond Ti:Sa laser. The effect was induced and simultaneously registered during the repetitive laser scanning of type I collagen (rat tail and bovine Achilles’ tendon), and bovine cornea. An irreversible increase in two-photon autofluorescence and a decrease in second harmonic generation intensities were associated with the collagen femtosecond laser photo-modification. Confocal spectral imaging revealed the formation of new fluorescent species. Controllable nonlinear photo-modification of collagen fibers and bovine cornea with ~2 µm spatial resolution was demonstrated.

© 2008 Optical Society of America

OCIS Codes
(170.5810) Medical optics and biotechnology : Scanning microscopy
(190.4180) Nonlinear optics : Multiphoton processes
(320.2250) Ultrafast optics : Femtosecond phenomena
(350.3390) Other areas of optics : Laser materials processing
(350.4600) Other areas of optics : Optical engineering

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: January 22, 2008
Revised Manuscript: March 4, 2008
Manuscript Accepted: March 19, 2008
Published: May 19, 2008

Virtual Issues
Vol. 3, Iss. 6 Virtual Journal for Biomedical Optics

Vladimir Hovhannisyan, Wen Lo, Chieh Hu, Shean-Jen Chen, and Chen Y. Dong, "Dynamics of femtosecond laser photo-modification of collagen fibers," Opt. Express 16, 7958-7968 (2008)

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