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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 16, Iss. 12 — Jun. 9, 2008
  • pp: 8581–8593

Fluorescence tomography characterization for sub-surface imaging with protoporphyrin IX

Dax Kepshire, Scott C. Davis, Hamid Dehghani, Keith D. Paulsen, and Brian W. Pogue  »View Author Affiliations


Optics Express, Vol. 16, Issue 12, pp. 8581-8593 (2008)
http://dx.doi.org/10.1364/OE.16.008581


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Abstract

Optical imaging of fluorescent objects embedded in a tissue simulating medium was characterized using non-contact based approaches to fluorescence remittance imaging (FRI) and sub-surface fluorescence diffuse optical tomography (FDOT). Using Protoporphyrin IX as a fluorescent agent, experiments were performed on tissue phantoms comprised of typical in-vivo tumor to normal tissue contrast ratios, ranging from 3.5:1 up to 10:1. It was found that tomographic imaging was able to recover interior inclusions with high contrast relative to the background; however, simple planar fluorescence imaging provided a superior contrast to noise ratio. Overall, FRI performed optimally when the object was located on or close to the surface and, perhaps most importantly, FDOT was able to recover specific depth information about the location of embedded regions. The results indicate that an optimal system for localizing embedded fluorescent regions should combine fluorescence reflectance imaging for high sensitivity and sub-surface tomography for depth detection, thereby allowing more accurate localization in all three directions within the tissue.

© 2008 Optical Society of America

OCIS Codes
(100.3190) Image processing : Inverse problems
(170.0110) Medical optics and biotechnology : Imaging systems
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.3010) Medical optics and biotechnology : Image reconstruction techniques
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.6960) Medical optics and biotechnology : Tomography

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: December 10, 2007
Revised Manuscript: March 5, 2008
Manuscript Accepted: March 14, 2008
Published: May 28, 2008

Virtual Issues
Vol. 3, Iss. 7 Virtual Journal for Biomedical Optics

Citation
Dax Kepshire, Scott C. Davis, Hamid Dehghani, Keith D. Paulsen, and Brian W. Pogue, "Fluorescence tomography characterization for sub-surface imaging with protoporphyrin IX," Opt. Express 16, 8581-8593 (2008)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-16-12-8581


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