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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 16, Iss. 12 — Jun. 9, 2008
  • pp: 8594–8603

A comparative study of living cell micromechanical properties by oscillatory optical tweezers

Ming-Tzo Wei, Angela Zaorski, Huseyin C. Yalcin, Jing Wang, Melissa Hallow, Samir N. Ghadiali, Arthur Chiou, and H. Daniel Ou-Yang  »View Author Affiliations


Optics Express, Vol. 16, Issue 12, pp. 8594-8603 (2008)
http://dx.doi.org/10.1364/OE.16.008594


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Abstract

Micromechanical properties of biological cells are crucial for cells functions. Despite extensive study by a variety of approaches, an understanding of the subject remains elusive. We conducted a comparative study of the micromechanical properties of cultured alveolar epithelial cells with an oscillatory optical tweezer-based cytorheometer. In this study, the frequency-dependent viscoelasticity of these cells was measured by optical trapping and forced oscillation of either a submicron endogenous intracellular organelle (intra-cellular) or a 1.5µm silica bead attached to the cytoskeleton through trans-membrane integrin receptors (extra-cellular). Both the storage modulus and the magnitude of the complex shear modulus followed weak power-law dependence with frequency. These data are comparable to data obtained by other measurement techniques. The exponents of power-law dependence of the data from the intra- and extra-cellular measurements are similar; however, the differences in the magnitudes of the moduli from the two measurements are statistically significant.

© 2008 Optical Society of America

OCIS Codes
(140.7010) Lasers and laser optics : Laser trapping
(170.1420) Medical optics and biotechnology : Biology
(170.1530) Medical optics and biotechnology : Cell analysis
(170.4520) Medical optics and biotechnology : Optical confinement and manipulation
(350.4855) Other areas of optics : Optical tweezers or optical manipulation

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: January 3, 2008
Revised Manuscript: May 19, 2008
Manuscript Accepted: May 22, 2008
Published: May 28, 2008

Virtual Issues
Vol. 3, Iss. 7 Virtual Journal for Biomedical Optics

Citation
Ming-Tzo Wei, Angela Zaorski, Huseyin C. Yalcin, Jing Wang, Melissa Hallow, Samir N. Ghadiali, Arthur Chiou, and H. Daniel Ou-Yang, "A comparative study of living cell micromechanical properties by oscillatory optical tweezers," Opt. Express 16, 8594-8603 (2008)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-16-12-8594


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