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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 16, Iss. 12 — Jun. 9, 2008
  • pp: 8670–8677

Simple and robust image-based autofocusing for digital microscopy

Siavash Yazdanfar, Kevin B. Kenny, Krenar Tasimi, Alex D. Corwin, Elizabeth L. Dixon, and Robert J. Filkins  »View Author Affiliations


Optics Express, Vol. 16, Issue 12, pp. 8670-8677 (2008)
http://dx.doi.org/10.1364/OE.16.008670


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Abstract

A simple image-based autofocusing scheme for digital microscopy is demonstrated that uses as few as two intermediate images to bring the sample into focus. The algorithm is adapted to a commercial inverted microscope and used to automate brightfield and fluorescence imaging of histopathology tissue sections.

© 2008 Optical Society of America

OCIS Codes
(110.3000) Imaging systems : Image quality assessment
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(180.0180) Microscopy : Microscopy

ToC Category:
Microscopy

History
Original Manuscript: April 15, 2008
Revised Manuscript: May 19, 2008
Manuscript Accepted: May 23, 2008
Published: May 28, 2008

Virtual Issues
Vol. 3, Iss. 7 Virtual Journal for Biomedical Optics

Citation
Siavash Yazdanfar, Kevin B. Kenny, Krenar Tasimi, Alex D. Corwin, Elizabeth L. Dixon, and Robert J. Filkins, "Simple and robust image-based autofocusing for digital microscopy," Opt. Express 16, 8670-8677 (2008)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-16-12-8670


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References

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