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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 16, Iss. 13 — Jun. 23, 2008
  • pp: 9290–9305

Adaptive optics for structured illumination microscopy

Delphine Débarre, Edward J. Botcherby, Martin J. Booth, and Tony Wilson  »View Author Affiliations

Optics Express, Vol. 16, Issue 13, pp. 9290-9305 (2008)

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We implement wave front sensor-less adaptive optics in a structured illumination microscope. We investigate how the image formation process in this type of microscope is affected by aberrations. It is found that aberrations can be classified into two groups, those that affect imaging of the illumination pattern and those that have no influence on this pattern. We derive a set of aberration modes ideally suited to this application and use these modes as the basis for an efficient aberration correction scheme. Each mode is corrected independently through the sequential optimisation of an image quality metric. Aberration corrected imaging is demonstrated using fixed fluorescent specimens. Images are further improved using differential aberration imaging for reduction of background fluorescence.

© 2008 Optical Society of America

OCIS Codes
(010.1080) Atmospheric and oceanic optics : Active or adaptive optics
(010.7350) Atmospheric and oceanic optics : Wave-front sensing
(180.2520) Microscopy : Fluorescence microscopy
(180.6900) Microscopy : Three-dimensional microscopy

ToC Category:
Adaptive Optics

Original Manuscript: April 11, 2008
Revised Manuscript: May 30, 2008
Manuscript Accepted: June 3, 2008
Published: June 9, 2008

Virtual Issues
Vol. 3, Iss. 7 Virtual Journal for Biomedical Optics

Delphine Débarre, Edward J. Botcherby, Martin J. Booth, and Tony Wilson, "Adaptive optics for structured illumination microscopy," Opt. Express 16, 9290-9305 (2008)

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