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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 16, Iss. 13 — Jun. 23, 2008
  • pp: 9357–9364

Targeted transfection of stem cells with sub-20 femtosecond laser pulses

Aisada Uchugonova, Karsten König, Rainer Bueckle, Andreas Isemann, and Gabriel Tempea  »View Author Affiliations


Optics Express, Vol. 16, Issue 13, pp. 9357-9364 (2008)
http://dx.doi.org/10.1364/OE.16.009357


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Abstract

Multiphoton microscopes have become important tools for non-contact sub-wavelength three-dimensional nanoprocessing of living biological specimens based on multiphoton ionization and plasma formation. Ultrashort laser pulses are required, however, dispersive effects limit the shortest pulse duration achievable at the focal plane. We report on a compact nonlinear laser scanning microscope with sub-20 femtosecond 75 MHz near infrared laser pulses for nanosurgery of human stem cells and two-photon high-resolution imaging. Single point illumination of the cell membrane was performed to induce a transient nanopore for the delivery of extracellular green fluorescent protein plasmids. Mean powers of less than 7 mW (<93 pJ) and low millisecond exposure times were found to be sufficient to transfect human pancreatic and salivary gland stem cells in these preliminary studies. Ultracompact sub-20 femtosecond laser microscopes may become optical tools for nanobiotechnology and nanomedicine including optical stem cell manipulation.

© 2008 Optical Society of America

OCIS Codes
(000.1430) General : Biology and medicine
(020.4180) Atomic and molecular physics : Multiphoton processes
(140.7090) Lasers and laser optics : Ultrafast lasers
(170.3890) Medical optics and biotechnology : Medical optics instrumentation
(140.3538) Lasers and laser optics : Lasers, pulsed
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: February 19, 2008
Revised Manuscript: April 1, 2008
Manuscript Accepted: June 9, 2008
Published: June 11, 2008

Virtual Issues
Vol. 3, Iss. 7 Virtual Journal for Biomedical Optics

Citation
Aisada Uchugonova, Karsten König, Rainer Bueckle, Andreas Isemann, and Gabriel Tempea, "Targeted transfection of stem cells with sub-20 femtosecond laser pulses," Opt. Express 16, 9357-9364 (2008)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-16-13-9357


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References

  1. M. Müller, J. Squier, R. Wolleschensky, U. Simon, and J. Brakenhoff, "Dispersion pre compensation for high-numerical aperture objectives," J. Microsc. 191, 141-150 (1997). [CrossRef]
  2. J. Jasapara and W. Rudolph, "Characterization of sub-10-fs pulse focussing with high numerical aperture microscope objectives," Opt. Lett. 24, 777-779 (1999). [CrossRef]
  3. S. Tang, T. B. Krasieva, Z. Chen, G. Tempea, and B. J. Tromberg, "Increasing efficiency of two-photon excited fluorescence and second harmonic generation using ultrashort laser pulses," Proc. SPIE 6089, 308-315 (2006).
  4. G. McConnell and E. Riss, "Two-photon laser scanning microscopy using photonic crystal fiber," J. Biomed. Opt. 9, 922-927 (2004). [CrossRef] [PubMed]
  5. R. Szipöcs, K, Ferencz, C. Spielmann, and F. Krausz, "Chirped multilayer coatings for broadband dispersion control in femtosecond lasers," Opt. Lett. 19, 201-203 (1994). [CrossRef] [PubMed]
  6. A. Stingl, C. Spielmann, F. Krausz, and R. Szipöcs, "Generation of 11-fs pulses from a Ti:sapphire laser without the use of prisms," Opt. Lett. 19, 204-206 (1994). [CrossRef] [PubMed]
  7. F. W. Helbing, G. Steinmeyer, J. Stenger, H. R. Telle, and U. Keller, "Carrier-envelope-offset dynamics and stabilization of femtosecond pulses," Appl. Phys. B 74, S35-S42 (2002).
  8. V. Venugopalan, A. Guerra, K. Nahen, and A. Vogel, "Role of laser-induced plasma formation in pulse cellular microsurgery and micromanipualtion," Phys. Rev. Lett. 88, 078103 (2002). [CrossRef] [PubMed]
  9. A. Vogel and V. Venugopalan, "Mechanism pulsed laser ablation of biological tissues," Chem Rev. 103, 577-644 (2003). [CrossRef] [PubMed]
  10. K. König, I. Riemann, and W. Fritzsche, "Nanodissection of human chromosomes with near infrared femtosecond laser pulses," Opt. Lett. 26, 819-821 (2001).
  11. U. K. Tirlapur and K. König, "Femtosecond near-infrared laser pulses as versatile non-invasive tool for intratissue nanoprocessing in plants without compromising viability," The Plant Journal 31, 365-374 (2002). [CrossRef] [PubMed]
  12. K. König, O. Krauss, and I. Riemann, "Intratissue surgery with 80 MHz nanojoule femtosecond laser pulses in the near infrared," Opt. Express 10, 171-176 (2002).
  13. F. Stracke, I. Riemann, and K. König, "Optical nanoinjection of macromolecules into vital cells," J. Photochem. Photobiol. B. 81, 136-142 (2005).
  14. K. König, I. Riemann, F. Stracke, and R. LeHarzic, "Nanoprocessing with nanojoule near-infrared femtosecond laser pulses," Med. Laser Appl. 20, 169-184 (2005). [CrossRef]
  15. A. Czaki, F. Garwe, A. Steinbrueck, G. Maubach, G. Festag, A. Weise, I. Riemann, K. König, and W. Fritzsche, "A parallel approach for sub-wavelength molecular surgery using gene-specific positioned metal nanoparticles as laser light antennas," Nanoletters 2, 247-253 (2007). [CrossRef]
  16. W. Watanabe, S. Matsunaga, T. Shimada, T. Higashi, K. Fukui, and K. Itoh, "Femtosecond laser disruption of mitochondria in living cells," Med. Laser Appl. 20, 185-191 (2005). [CrossRef]
  17. V. Kohli, A. Elezzabi, and J. P. Acker, "Cell nanosurgery using ultrashort (femtosecond) laser pulses: applications to membrane surgery and cell isolation," Lasers Surg. Med. 37, 227-230 (2005). [CrossRef] [PubMed]
  18. L. Sacconi, I. Tolic-Norrelykke, R. Antolini, and F. S. Pavone, "Combined intracellualar three-dimensional imaging and selective nanosurgery by a nonlinear microscope," J. Biomed. Opt. 10, 14002 (2005). [CrossRef] [PubMed]
  19. M. F. Yanik, H. Cinar, H. N. Cinar, A. D. Chisholm, Y. Jin, and A. Ben-Yakar, "Nanosurgery: functional regeneration after laser axetomy," Nature 432, 822 (2004). [CrossRef] [PubMed]
  20. U. K. Tirpalur and K. König, "Targeted transfection by femtosecond laser," Nature 418, 4295-4298 (2002).
  21. X. Tsampoula, V. Garces-Chavez, M. Comrie, D. J. Stevenson, B. Agate, C. T. A. Brown, F. Gunn-Moore, and K. Dholakia, "Femtosecond cellular transfection using a nondiffracting light beam," Appl. Phys. Lett. 91, 053902 (2007). [CrossRef]
  22. D. Stevenson, B. Agate, X. Tsampoula, P. Fischer, C. T. A. Brown, W. Sibbett, A. Riches, F. Gunn-Moore, and K. Dholakia, "Femtosecond optical transfection of cells: viability and efficiency," Opt. Express 14, 7125-7133 (2006). [CrossRef] [PubMed]
  23. A. Hamm, N. Krott, I. Breibach, R. Blindt, and A. K. Bosserhof, "Efficient transfection method for primary cells," Tissue Engineering 8, 1-13 (2002). [CrossRef]
  24. H. Haleem-Smith, A. Derfoul, C. Okafor, R. Tuli, D. Olsen, D. J. Hall, and R. S. Tuan, "Optimization of high-efficiency transfection of adult human mesenchymal stem cells in vitro," Mol. Biotech. 3, 09-19 (2005). [CrossRef]
  25. A. M. Larson and A. T. Yeh, "Ex vivo characterization of sub-10 fs pulses," Opt. Lett. 31,1681-1683 (2006). [CrossRef] [PubMed]
  26. G. Tempea, B. Považay, A. Assion, A. Isemann, W. Pervak, M. Kempe, A. Stingl, and W. Drexler, "Undistored delivery of sub-15 fs pulses via high-numerical-aperture microscope objectives," Proc. SPIE 6442, 64420P-1-5 (2007). [CrossRef]
  27. C. Kruse, M. Birth, J. Rohwedel, K. Assmuth, A. Goepel, and T. Wedel, "Pluripotency of adult stem cells derived from human and rat pancreas," Appl. Phys. A. 79, 1617-1624 (2004). [CrossRef]
  28. C. Kruse, J. Kajahn, A. E. Petschnik, A. Maas, E. Klink, D. H. Rapoport, and T. Wedel, "Adult pancreatic stem/progenitor cells spontaneously differentiate in vitro into multiple cell lineages and form teratoma-like structures," Annals of Anatomy 188, 503-517 (2006). [CrossRef] [PubMed]

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