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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 16, Iss. 13 — Jun. 23, 2008
  • pp: 9534–9548

Cell tracking and detection of molecular expression in live cells using lipid-enclosed CdSe quantum dots as contrast agents for epi-third harmonic generation microscopy

Chieh-Feng Chang, Chao-Yu Chen, Fu-Hsiung Chang, Shih-Peng Tai, Cheng-Ying Chen, Che-Hang Yu, Yi-Bing Tseng, Tsung-Han Tsai, I-Shuo Liu, Wei-Fang Su , and Chi-Kuang Sun  »View Author Affiliations

Optics Express, Vol. 16, Issue 13, pp. 9534-9548 (2008)

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We demonstrated that lipid-enclosed CdSe quantum dots (LEQDs) can function as versatile contrast agents in epi-detection third harmonic generation (THG) microscopy for biological applications in vivo. With epi-THG intensities 20 times stronger than corresponding fluorescence intensities from the same LEQDs under the same conditions of energy absorption, such high brightness LEQDs were proved for the abilities of cell tracking and detection of specific molecular expression in live cancer cells. Using nude mice as an animal model, the distribution of LEQD-loaded tumor cells deep in subcutaneous tissues were imaged with high THG contrast. This is the first demonstration that THG contrast can be manipulated in vivo with nanoparticles. By linking LEQDs with anti-Her2 antibodies, the expression of Her2/neu receptors in live breast cancer cells could also be easily detected through THG. Compared with fluorescence modalities, the THG modality also provides the advantage of no photobleaching and photoblinking effects. Combined with a high penetration 1230 nm laser, these novel features make LEQDs excellent THG contrast agents for in vivo deep-tissue imaging in the future.

© 2008 Optical Society of America

OCIS Codes
(180.5810) Microscopy : Scanning microscopy
(180.6900) Microscopy : Three-dimensional microscopy
(190.4160) Nonlinear optics : Multiharmonic generation

ToC Category:

Original Manuscript: February 22, 2008
Revised Manuscript: May 26, 2008
Manuscript Accepted: May 27, 2008
Published: June 13, 2008

Virtual Issues
Vol. 3, Iss. 7 Virtual Journal for Biomedical Optics

Chieh-Feng Chang, Chao-Yu Chen, Fu-Hsiung Chang, Shih-Peng Tai, Cheng-Ying Chen, Che-Hang Yu, Yi-Bing Tseng, Tsung-Han Tsai, I-Shuo Liu, Wei-Fang Su, and Chi-Kuang Sun, "Cell tracking and detection of molecular expression in live cells using lipid-enclosed CdSe quantum dots as contrast agents for epi-third harmonic generation microscopy," Opt. Express 16, 9534-9548 (2008)

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