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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 16, Iss. 13 — Jun. 23, 2008
  • pp: 9614–9621

STED microscopy with a supercontinuum laser source

Dominik Wildanger, Eva Rittweger, Lars Kastrup, and Stefan W. Hell  »View Author Affiliations


Optics Express, Vol. 16, Issue 13, pp. 9614-9621 (2008)
http://dx.doi.org/10.1364/OE.16.009614


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Abstract

We report on a straightforward yet powerful implementation of stimulated emission depletion (STED) fluorescence microscopy providing subdiffraction resolution in the far-field. Utilizing the same supercontinuum pulsed laser source both for excitation and STED, this implementation of STED microscopy avoids elaborate preparations of laser pulses and conveniently provides multicolor imaging. Operating at pulse repetition rates around 1 MHz, it also affords reduced photobleaching rates by allowing the fluorophore to relax from excitable metastable dark states involved in photodegradation. The imaging of dense nanoparticles and of the microtubular network of mammalian cells evidences a spatial resolution of 30–50 nm in the focal plane, i.e. by a factor of 8–9 beyond the diffraction barrier.

© 2008 Optical Society of America

OCIS Codes
(180.1790) Microscopy : Confocal microscopy
(180.2520) Microscopy : Fluorescence microscopy
(320.6629) Ultrafast optics : Supercontinuum generation

ToC Category:
Microscopy

History
Original Manuscript: May 6, 2008
Revised Manuscript: May 30, 2008
Manuscript Accepted: May 30, 2008
Published: June 13, 2008

Virtual Issues
Vol. 3, Iss. 7 Virtual Journal for Biomedical Optics

Citation
Dominik Wildanger, Eva Rittweger, Lars Kastrup, and Stefan W. Hell, "STED microscopy with a supercontinuum laser source," Opt. Express 16, 9614-9621 (2008)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-16-13-9614


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References

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