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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 16, Iss. 13 — Jun. 23, 2008
  • pp: 9811–9820

Digital holography of total internal reflection

William M. Ash III and Myung K. Kim  »View Author Affiliations

Optics Express, Vol. 16, Issue 13, pp. 9811-9820 (2008)

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We introduce a new microscopy technique termed total internal reflection holographic microscopy (TIRHM). Quantitative phase microscopy by digital holography is used to image the phase profile of light in total internal reflection, which is modulated by the materials present on or near the surface of internal reflection. The imaging characteristics are theoretically modeled and imaging capabilities are experimentally demonstrated.

© 2008 Optical Society of America

OCIS Codes
(110.0180) Imaging systems : Microscopy
(170.0180) Medical optics and biotechnology : Microscopy
(170.1530) Medical optics and biotechnology : Cell analysis
(180.3170) Microscopy : Interference microscopy
(090.1995) Holography : Digital holography
(090.5694) Holography : Real-time holography

ToC Category:

Original Manuscript: May 13, 2008
Revised Manuscript: June 9, 2008
Manuscript Accepted: June 15, 2008
Published: June 19, 2008

Virtual Issues
Vol. 3, Iss. 7 Virtual Journal for Biomedical Optics

William M. Ash and Myung K. Kim, "Digital holography of total internal reflection," Opt. Express 16, 9811-9820 (2008)

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