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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 16, Iss. 13 — Jun. 23, 2008
  • pp: 9857–9868

Enzymatic degradation of human skin dermis revealed by fluorescence and reflectance spectroscopy

Ye Yuan and Patricia Relue  »View Author Affiliations


Optics Express, Vol. 16, Issue 13, pp. 9857-9868 (2008)
http://dx.doi.org/10.1364/OE.16.009857


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Abstract

Dermis is the major source of the fluorescence and light scattering of skin. Tumor-induced degradation of the dermis is expected to change the fluorescence and light scattering properties of skin. To investigate how these fluorescence and light scattering properties are changed, human skin dermis was degraded with enzymes to mimic tumor invasion. The enzymatic erosion process was investigated with fluorescence and reflectance spectroscopy. Dermis degradation by the enzymes resulted in a decrease in fluorescence emission and light scattering in the dermis. Fluorescence anisotropy, however, could not detect the change in the dermis induced by the enzyme treatments.

© 2008 Optical Society of America

OCIS Codes
(170.4580) Medical optics and biotechnology : Optical diagnostics for medicine
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence
(170.6510) Medical optics and biotechnology : Spectroscopy, tissue diagnostics
(170.6930) Medical optics and biotechnology : Tissue

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: December 5, 2007
Revised Manuscript: March 3, 2008
Manuscript Accepted: June 9, 2008
Published: June 20, 2008

Virtual Issues
Vol. 3, Iss. 7 Virtual Journal for Biomedical Optics

Citation
Ye Yuan and Patricia Relue, "Enzymatic degradation of human skin dermis revealed by fluorescence and reflectance spectroscopy," Opt. Express 16, 9857-9868 (2008)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-16-13-9857


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