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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 16, Iss. 13 — Jun. 23, 2008
  • pp: 9884–9894

In vivo nanosecond laser axotomy: cavitation dynamics and vesicle transport

G. Nageswara Rao, Sucheta S. Kulkarni, Sandhya P. Koushika, and Kaustubh R. Rau  »View Author Affiliations

Optics Express, Vol. 16, Issue 13, pp. 9884-9894 (2008)

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Nanosecond laser pulses (λ=355 nm) were used to cut mechanosensory neurons in Caenorhabditis elegans and motorneurons in Drosophila melanogaster larvae. A pulse energy range of 0.8–1.2 µJ and <20 pulses in single shot mode were sufficient to generate axonal cuts. Viability post-surgery was >95% for C. elegans and 60% for Drosophila. Cavitation bubble dynamics generated due to laser-induced plasma formation were observed in vivo by time-resolved imaging in both organisms. Bubble oscillations were severely damped in vivo and cavitation dynamics were complete within 100 ns in C. elegans and 800 ns in Drosophila. We report the use of this system to study axonal transport for the first time and discuss advantages of nanosecond lasers compared to femtosecond sources for such procedures.

© 2008 Optical Society of America

OCIS Codes
(140.3440) Lasers and laser optics : Laser-induced breakdown
(170.1020) Medical optics and biotechnology : Ablation of tissue
(170.6920) Medical optics and biotechnology : Time-resolved imaging

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: March 4, 2008
Revised Manuscript: June 1, 2008
Manuscript Accepted: June 2, 2008
Published: June 20, 2008

Virtual Issues
Vol. 3, Iss. 7 Virtual Journal for Biomedical Optics

G. Nageswara Rao, Sucheta S. Kulkarni, Sandhya P. Koushika, and Kaustubh R. Rau, "In vivo nanosecond laser axotomy: cavitation dynamics and vesicle transport," Opt. Express 16, 9884-9894 (2008)

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