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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 16, Iss. 13 — Jun. 23, 2008
  • pp: 9884–9894

In vivo nanosecond laser axotomy: cavitation dynamics and vesicle transport

G. Nageswara Rao, Sucheta S. Kulkarni, Sandhya P. Koushika, and Kaustubh R. Rau  »View Author Affiliations


Optics Express, Vol. 16, Issue 13, pp. 9884-9894 (2008)
http://dx.doi.org/10.1364/OE.16.009884


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Abstract

Nanosecond laser pulses (λ=355 nm) were used to cut mechanosensory neurons in Caenorhabditis elegans and motorneurons in Drosophila melanogaster larvae. A pulse energy range of 0.8–1.2 µJ and <20 pulses in single shot mode were sufficient to generate axonal cuts. Viability post-surgery was >95% for C. elegans and 60% for Drosophila. Cavitation bubble dynamics generated due to laser-induced plasma formation were observed in vivo by time-resolved imaging in both organisms. Bubble oscillations were severely damped in vivo and cavitation dynamics were complete within 100 ns in C. elegans and 800 ns in Drosophila. We report the use of this system to study axonal transport for the first time and discuss advantages of nanosecond lasers compared to femtosecond sources for such procedures.

© 2008 Optical Society of America

OCIS Codes
(140.3440) Lasers and laser optics : Laser-induced breakdown
(170.1020) Medical optics and biotechnology : Ablation of tissue
(170.6920) Medical optics and biotechnology : Time-resolved imaging

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: March 4, 2008
Revised Manuscript: June 1, 2008
Manuscript Accepted: June 2, 2008
Published: June 20, 2008

Virtual Issues
Vol. 3, Iss. 7 Virtual Journal for Biomedical Optics

Citation
G. Nageswara Rao, Sucheta S. Kulkarni, Sandhya P. Koushika, and Kaustubh R. Rau, "In vivo nanosecond laser axotomy: cavitation dynamics and vesicle transport," Opt. Express 16, 9884-9894 (2008)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-16-13-9884


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References

  1. M. F. Yanik, H. Cinar, H. N. Cinar, A. D. Chisholm, Y. Jin, and A. Ben-Yakar, "Functional regeneration after laser axotomy," Nature 432,882 (2004). [CrossRef]
  2. S. H. Chung, D. A. Clark, C. V. Gabel, E. Mazur, and A. D. Samuel,"The role of the AFD neuron in C. elegans thermotaxis analyzed using femtosecond laser ablation," BMC Neuroscience 7, DOI:10.1186/1471-2202-7-30 (2006). [CrossRef] [PubMed]
  3. Z. Wu, A. Ghosh-Roy, M. F. Yanik, J. Z. Zhang, Y. Jin, and A. D. Chisholm, "Caenorhabditis elegans neuronal regeneration is influenced by life stage, ephrin signaling and synaptic branching," PNAS 104,15132-15137 (2007). [CrossRef] [PubMed]
  4. P. Weiss and H. B. Hiscoe,"Experiments on the mechanism of nerve growth," J. Exp. Zool. 107315-395 (1948). [CrossRef] [PubMed]
  5. R. V. Barkus, O. Klyachko, D. Horiuchi B. J. Dickson and W. M. Saxton, "Identification of an Axonal Kinesin-3 Motor for Fast Anterograde Vesicle Transport that Facilitates Retrograde Transport of Neuropeptides," Mol. Biol. Cell 19,274-283 (2008). [CrossRef]
  6. H. Erez, G. Malkinson, M. Prager-Khoutorsky, C. I. De Zeeuw, C. C. Hoogenraad, and M. E. Spira, "Formation of microtubule-based traps controls the sorting and concentration of vesicles to restricted sites of regenerating neurons after axotomy," J. Cell Biol. 176,497-507 (2007). [CrossRef] [PubMed]
  7. M. S. Hutson and X. Ma, "Plasma and cavitation dynamics during pulsed laser microsurgery in vivo," Phys. Rev. Lett. 99,158104 (2007). [CrossRef] [PubMed]
  8. T. R. Mahoney, Q. Liu, T. Itoh, S. Luo, G. Hadwiger, R. Vincent, Z-W. Wang, M. Fukuda, and M. L. Nonet, "Regulation of synaptic transmission by RAB-3 and RAB-27 in Caenorhabditis elegans," Mol. Biol. Cell 17,2617-2625 (2006). [CrossRef] [PubMed]
  9. M. L. Nonet, "Visualization of synaptic specializations in live C. elegans with synaptic vesicle protein-GFP fusions," J. Neurosci. Methods 89,33-40 (1999). [CrossRef] [PubMed]
  10. T. Lee and L. Luo, "Mosaic analysis with a repressible cell marker for studies of gene function in neuronal morphogenesis," Neuron 22,451-61 (1999). [CrossRef] [PubMed]
  11. A. Mahr and H. Aberle, "The expression pattern of the Drosophila vesicular glutamate transporter: A marker protein for motoneurons and glutamatergic centers in the brain, Gene Expr. Patterns 6,299-309 (2006). [CrossRef]
  12. A. V. Cherian and K. R. Rau, "Pulsed laser-induced damage in rat corneas: time-resolved imaging of physical effects and acute biological response," J. Biomed. Opt. 13,024009 (2008). [CrossRef] [PubMed]
  13. M. F. Yanik, H. Cinar, H. N. Cinar, A. Gibby, A. D. Chisholm, Y. Jin, and A. Ben-Yakar, "Nerve regeneration in Caenorhabditis elegans after femtosecond laser axotomy," IEEE J. Quantum Electron. 12,1283-1291 (2006). [CrossRef]
  14. V. Venugopalan, A. GuerraIII, K. Nahen, and A. Vogel, "Role of Laser-Induced Plasma Formation in Pulsed Cellular Microsurgery and Micromanipulation," Phys. Rev. Lett. 88,078103 (2002). [CrossRef] [PubMed]
  15. U. K. Tirlapur and K. Konig, "Targeted transfection by femtosecond laser," Nature 418,290-291 (2002). [CrossRef] [PubMed]
  16. W. Watanabe and N. Arakawa, "Femtosecond laser disruption of subcellular organelles in a living cell," Opt. Express 12,4203-4213 (2004). [CrossRef] [PubMed]
  17. A. Heisterkamp, I. Zaharieva Maxwell, E. Mazur, J. M. Underwood, J. A. Nickerson, S. Kumar, and D. E. Ingber, "Pulse energy dependence of subcellular dissection by femtosecond laser pulses," Opt. Express 13,3690-3696 (2005). [CrossRef] [PubMed]
  18. V. Magidson, J. Loncarek, P. Hergert, C. L. Reider, and A. Khodjakov,"Laser Microsurgery in the GFP Era: A Cell Biologist???s Perspective," Methods Cell Biol. 82,239-266, (2007). [PubMed]
  19. W. Grill, P. Gonczy, E. H. K. Stelzer, and A. A. Hyman "Polarity controls forces governing asymmetric spindle positioning in the Caenorhabditis elegans embryo," Nature 409,630-633 (2001). [CrossRef] [PubMed]
  20. M. S. Hutson, Y. Tokutake, M.-S. Chang, J.W. Bloor, S. Venakides, D. P. Kiehart, and G. S. Edwards "Forces for morphogenesis investigated with laser-microsurgery and quantitative modeling," Science 300,145-149 (2003). [CrossRef] [PubMed]
  21. F. Bourgeois and A. Ben-Yakar, "Femtosecond laser nanoaxotomy properties and their effect on axonal recovery in C. elegans," Opt. Express 15,8521-8531 (2007). [CrossRef] [PubMed]
  22. J. J. Fernandes and H. Keshishian "Nerve-muscle interactions during flight muscle development in Drosophila," Development,  125,1769-1779 (1998). [PubMed]
  23. K. R. Rau, P. A. Quinto-Su, A. Hellman, and V. Venugopalan, "Pulsed laser microbeam-induced cell lysis: timeresolved imaging and analysis of hydrodynamic effects," Biophys. J. 91,317-329 (2006). [CrossRef] [PubMed]
  24. A. Vogel, M. R. C. Capon, M. N. Asiyo-Vogel, and R. Birngruber, "Intraocular photodisruption with picosecond and nanosecond laser pulses: Tissue effects in cornea, lens, and retina," Invest. Ophthalmol. Visual Sci. 35,3032-3044 (1994).
  25. A. Vogel, N. Linz, S. Freidank, and G. Paltauf, "Femtosecond-Laser-Induced Nanocavitation in Water: Implications for Optical Breakdown Threshold and Cell Surgery," Phys. Rev. Lett. 100,038102 (2008). [CrossRef] [PubMed]

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