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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 16, Iss. 13 — Jun. 23, 2008
  • pp: 9996–10005

Optics InfoBase > Optics Express > Volume 16 > Issue 13 > Page 9996

Miniaturized probe for femtosecond laser microsurgery and two-photon imaging

Christopher L. Hoy, Nicholas J. Durr, Pengyuan Chen, Wibool Piyawattanametha, Hyejun Ra, Olav Solgaard, and Adela Ben-Yakar  »View Author Affiliations


Optics Express, Vol. 16, Issue 13, pp. 9996-10005 (2008)
http://dx.doi.org/10.1364/OE.16.009996


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Abstract

Combined two-photon fluorescence microscopy and femtosecond laser microsurgery has many potential biomedical applications as a powerful “seek-and-treat” tool. Towards developing such a tool, we demonstrate a miniaturized probe which combines these techniques in a compact housing. The device is 10×15×40 mm3 in size and uses an air-core photonic crystal fiber to deliver femtosecond laser pulses at 80 MHz repetition rate for imaging and 1 kHz for microsurgery. A fast two-axis microelectromechanical system scanning mirror is driven at resonance to produce Lissajous beam scanning at 10 frames per second. Field of view is 310 µm in diameter and the lateral and axial resolutions are 1.64 µm and 16.4 µm, respectively. Combined imaging and microsurgery is demonstrated using live cancer cells.

© 2008 Optical Society of America

OCIS Codes
(170.1020) Medical optics and biotechnology : Ablation of tissue
(170.2150) Medical optics and biotechnology : Endoscopic imaging
(190.4180) Nonlinear optics : Multiphoton processes

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: May 2, 2008
Revised Manuscript: June 8, 2008
Manuscript Accepted: June 10, 2008
Published: June 20, 2008

Virtual Issues
Vol. 3, Iss. 7 Virtual Journal for Biomedical Optics

Citation
Christopher L. Hoy, Nicholas J. Durr, Pengyuan Chen, Wibool Piyawattanametha, Hyejun Ra, Olav Solgaard, and Adela Ben-Yakar, "Miniaturized probe for femtosecond laser microsurgery and two-photon imaging," Opt. Express 16, 9996-10005 (2008)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-16-13-9996


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