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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 16, Iss. 14 — Jul. 7, 2008
  • pp: 10364–10371

Simultaneous multifocal, multiphoton, photon counting microscopy

Ramón Carriles, Kraig E. Sheetz, Erich E. Hoover, Jeff A. Squier, and Virginijus Barzda  »View Author Affiliations


Optics Express, Vol. 16, Issue 14, pp. 10364-10371 (2008)
http://dx.doi.org/10.1364/OE.16.010364


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Abstract

We demonstrate a novel multifocal, multiphoton microscope that is capable of simultaneous dynamic imaging of multiple focal planes. We show for the first time that multimodal, multiphoton images excited with orthogonal polarizations can be acquired simultaneously in both the transmission and epi directions.

© 2008 Optical Society of America

OCIS Codes
(030.5260) Coherence and statistical optics : Photon counting
(170.0180) Medical optics and biotechnology : Microscopy
(180.5810) Microscopy : Scanning microscopy
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:
Microscopy

History
Original Manuscript: May 2, 2008
Revised Manuscript: June 2, 2008
Manuscript Accepted: June 10, 2008
Published: June 26, 2008

Virtual Issues
Vol. 3, Iss. 8 Virtual Journal for Biomedical Optics

Citation
Ramón Carriles, Kraig E. Sheetz, Erich E. Hoover, Jeff A. Squier, and Virginijus Barzda, "Simultaneous multifocal, multiphoton, photon counting microscopy," Opt. Express 16, 10364-10371 (2008)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-16-14-10364


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References

  1. P. Prabhat, S. Ram, E. S. Ward, and R. J. Ober, "Simultaneous imaging of different focal planes in fluorescence microscopy for the study of cellular dynamics in three dimensions," IEEE Trans. Nanobiosci. 3, 237-242 (2004). [CrossRef]
  2. B. Rózsa, G. Katona, E. S. Vizi, Z. Várallyay, A. Sághy, L. Valenta, P. Maák, J. Fekete, A. Bányász, and R. Szipöcs, "Random access three-dimensional two-photon microscopy," Appl. Opt. 46, 1860-1865 (2007). [CrossRef] [PubMed]
  3. W. Amir, R. Carriles, E. E. Hoover, T. A. Planchon, C. G. Durfee, and J. A. Squier, "Simultaneous imaging of multiple focal planes using a two-photon scanning microscope," Opt. Lett. 32, 1731-1733 (2007). [CrossRef] [PubMed]
  4. J. O. Hamblen, T. S. Hall, and M. D. Furman, Rapid Prototyping of Digital Systems: Quartus II Edition (Springer, 2006).
  5. R. Carriles, E. E. Hoover, W. Amir, and J. A. Squier, "Simultaneous imaging of multiple focal planes in scanning two-photon absorption microscope by photon counting," Proc. SPIE 6771, 67710C-2 - 67710C-8 (2007).
  6. J. Bewersdorf, R. Pick, and S. W. Hell, "Multifocal multiphoton microscopy," Opt. Lett. 23, 655-657 (1998). [CrossRef]
  7. A. H. Buist, M. Müller, J. Squier, and G. J. Brakenhoff, "Real time two-photon absorption microscopy using multi point excitation," J. Microsc. 192, 217-226 (1998). [CrossRef]
  8. M. Fricke, and T. Nielsen, "Two-dimensional imaging without scanning by multifocal multiphoton microscopy," Appl. Opt. 44, 2984-2988 (2005). [CrossRef] [PubMed]
  9. D. J. Gallant, B. Bouchet, and P. M. Baldwin, "Microscopy of starch: evidence of a new level of granule organization," Carbohydr. Polym. 32, 177-191 (1997). [CrossRef]
  10. G. Mizutani, Y. Sonoda, H. Sano, M. Sakamoto, T. Takahashi, and S. Ushioda, "Detection of starch granules in a living plant by optical second harmonic microscopy," J. Lumin. 87-89, 824-826 (2000). [CrossRef]
  11. S. W. Chu, I. H. Chen, T. M. Liu, P. C. Chen, C. K. Sun, and B. L. Lin, "Multimodal nonlinear spectral microscopy based on a femtosecond Cr:forsterite laser," Opt. Lett. 26, 1909-1911 (2001). [CrossRef]
  12. K. N. A. Thayil, E. J. Gualda, S. Psilodimitrakopoulos, I. G. Cormack, I. Amat-Roldán, M. Mathew, D. Artigas, and P. Loza-Alvarez, "Starch-based backwards SHG for in situ MEFISTO pulse characterization in multiphoton microscopy," J. Microsc. 230, 70-75 (2008). [CrossRef]
  13. R. Cisek, N. Prent, C. Greenhalgh, D. Sandkuijl, A. Tuer, A. Major, and V. Barzda, "Multicontrast nonlinear imaging microscopy," in Biochemical Applications of Nonlinear Optical Spectroscopy, V. V. Yakovlev, ed. (CRC Press, in press).

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