OSA's Digital Library

Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 16, Iss. 14 — Jul. 7, 2008
  • pp: 10364–10371

Simultaneous multifocal, multiphoton, photon counting microscopy

Ramón Carriles, Kraig E. Sheetz, Erich E. Hoover, Jeff A. Squier, and Virginijus Barzda  »View Author Affiliations

Optics Express, Vol. 16, Issue 14, pp. 10364-10371 (2008)

View Full Text Article

Enhanced HTML    Acrobat PDF (293 KB)

Browse Journals / Lookup Meetings

Browse by Journal and Year


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools



We demonstrate a novel multifocal, multiphoton microscope that is capable of simultaneous dynamic imaging of multiple focal planes. We show for the first time that multimodal, multiphoton images excited with orthogonal polarizations can be acquired simultaneously in both the transmission and epi directions.

© 2008 Optical Society of America

OCIS Codes
(030.5260) Coherence and statistical optics : Photon counting
(170.0180) Medical optics and biotechnology : Microscopy
(180.5810) Microscopy : Scanning microscopy
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:

Original Manuscript: May 2, 2008
Revised Manuscript: June 2, 2008
Manuscript Accepted: June 10, 2008
Published: June 26, 2008

Virtual Issues
Vol. 3, Iss. 8 Virtual Journal for Biomedical Optics

Ramón Carriles, Kraig E. Sheetz, Erich E. Hoover, Jeff A. Squier, and Virginijus Barzda, "Simultaneous multifocal, multiphoton, photon counting microscopy," Opt. Express 16, 10364-10371 (2008)

Sort:  Author  |  Year  |  Journal  |  Reset  


  1. P. Prabhat, S. Ram, E. S. Ward, and R. J. Ober, "Simultaneous imaging of different focal planes in fluorescence microscopy for the study of cellular dynamics in three dimensions," IEEE Trans. Nanobiosci. 3, 237-242 (2004). [CrossRef]
  2. B. Rózsa, G. Katona, E. S. Vizi, Z. Várallyay, A. Sághy, L. Valenta, P. Maák, J. Fekete, A. Bányász, and R. Szipöcs, "Random access three-dimensional two-photon microscopy," Appl. Opt. 46, 1860-1865 (2007). [CrossRef] [PubMed]
  3. W. Amir, R. Carriles, E. E. Hoover, T. A. Planchon, C. G. Durfee, and J. A. Squier, "Simultaneous imaging of multiple focal planes using a two-photon scanning microscope," Opt. Lett. 32, 1731-1733 (2007). [CrossRef] [PubMed]
  4. J. O. Hamblen, T. S. Hall, and M. D. Furman, Rapid Prototyping of Digital Systems: Quartus II Edition (Springer, 2006).
  5. R. Carriles, E. E. Hoover, W. Amir, and J. A. Squier, "Simultaneous imaging of multiple focal planes in scanning two-photon absorption microscope by photon counting," Proc. SPIE 6771, 67710C-2 - 67710C-8 (2007).
  6. J. Bewersdorf, R. Pick, and S. W. Hell, "Multifocal multiphoton microscopy," Opt. Lett. 23, 655-657 (1998). [CrossRef]
  7. A. H. Buist, M. Müller, J. Squier, and G. J. Brakenhoff, "Real time two-photon absorption microscopy using multi point excitation," J. Microsc. 192, 217-226 (1998). [CrossRef]
  8. M. Fricke, and T. Nielsen, "Two-dimensional imaging without scanning by multifocal multiphoton microscopy," Appl. Opt. 44, 2984-2988 (2005). [CrossRef] [PubMed]
  9. D. J. Gallant, B. Bouchet, and P. M. Baldwin, "Microscopy of starch: evidence of a new level of granule organization," Carbohydr. Polym. 32, 177-191 (1997). [CrossRef]
  10. G. Mizutani, Y. Sonoda, H. Sano, M. Sakamoto, T. Takahashi, and S. Ushioda, "Detection of starch granules in a living plant by optical second harmonic microscopy," J. Lumin. 87-89, 824-826 (2000). [CrossRef]
  11. S. W. Chu, I. H. Chen, T. M. Liu, P. C. Chen, C. K. Sun, and B. L. Lin, "Multimodal nonlinear spectral microscopy based on a femtosecond Cr:forsterite laser," Opt. Lett. 26, 1909-1911 (2001). [CrossRef]
  12. K. N. A. Thayil, E. J. Gualda, S. Psilodimitrakopoulos, I. G. Cormack, I. Amat-Roldán, M. Mathew, D. Artigas, and P. Loza-Alvarez, "Starch-based backwards SHG for in situ MEFISTO pulse characterization in multiphoton microscopy," J. Microsc. 230, 70-75 (2008). [CrossRef]
  13. R. Cisek, N. Prent, C. Greenhalgh, D. Sandkuijl, A. Tuer, A. Major, and V. Barzda, "Multicontrast nonlinear imaging microscopy," in Biochemical Applications of Nonlinear Optical Spectroscopy, V. V. Yakovlev, ed. (CRC Press, in press).

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited