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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 16, Iss. 14 — Jul. 7, 2008
  • pp: 10440–10454

Improved accuracy in time-resolved diffuse reflectance spectroscopy

Erik Alerstam, Stefan Andersson-Engels, and Tomas Svensson  »View Author Affiliations

Optics Express, Vol. 16, Issue 14, pp. 10440-10454 (2008)

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Significant improvements in the accuracy of time-resolved diffuse reflectance spectroscopy are reached by using a Monte Carlo scheme for evaluation of measured photon time-of-flight distributions. The use of time-resolved diffusion theory of photon migration, being the current standard scheme for data evaluation, is shown defective. In particular, the familiar problem sometimes referred to as absorption-to-scattering coupling or crosstalk, is identified as an error related to the breakdown of the diffusion approximation. These systematic errors are investigated numerically using Monte Carlo simulations, and their influence on data evaluation of experimental recordings are accurately predicted. The proposed Monte Carlo-based data evaluation avoids these errors, and can be used for routine data evaluation. The accuracy and reproducibility of both MC and diffusion modeling are investigated experimentally using the MEDPHOT set of solid tissue-simulating phantoms, and provides convincing arguments that Monte Carlo-based evaluation is crucial in important ranges of optical properties. In contrast to diffusion-based evaluation, the Monte Carlo scheme results in optical properties consistent with phantom design. Since the MEDPHOT phantoms are used for international comparisons and performance assessment, the performed characterization is carefully reported.

© 2008 Optical Society of America

OCIS Codes
(160.4760) Materials : Optical properties
(170.5280) Medical optics and biotechnology : Photon migration
(170.6510) Medical optics and biotechnology : Spectroscopy, tissue diagnostics
(170.7050) Medical optics and biotechnology : Turbid media
(290.1990) Scattering : Diffusion
(300.6500) Spectroscopy : Spectroscopy, time-resolved

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: April 10, 2008
Revised Manuscript: May 8, 2008
Manuscript Accepted: May 13, 2008
Published: June 27, 2008

Virtual Issues
Vol. 3, Iss. 8 Virtual Journal for Biomedical Optics

Erik Alerstam, Stefan Andersson-Engels, and Tomas Svensson, "Improved accuracy in time-resolved diffuse reflectance spectroscopy," Opt. Express 16, 10440-10454 (2008)

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  1. B. Chance, S. Nioka, J. Kent, K. Mccully, M. Fountain, R. Greenfeld, and G. Holtom, "Time-resolved spectroscopy of hemoglobin and myoglobin in resting and ischemic muscle," Anal. Biochem. 174, 698-707 (1988). [CrossRef] [PubMed]
  2. B. Chance, J. Leigh, H. Miyake, D. Smith, S. Nioka, R. Greenfeld, M. Finander, K. Kaufmann, W. Levy, M. Young, P. Cohen, H. Yoshioka, and R. Boretsky, "Comparison of time-resolved and time-unresolved measurements of deoxyhemoglobin in brain," P. Natl. Acad. Sci. USA 85, 4971-4975 (1988). [CrossRef]
  3. A. Pifferi, J. Swartling, E. Chikoidze, A. Torricelli, P. Taroni, A. Bassi, S. Andersson-Engels, and R. Cubeddu, "Spectroscopic time-resolved diffuse reflectance and transmittance measurements of the female breast at different interfiber distances," J. Biomed. Opt. 9, 1143-1151 (2004). [CrossRef] [PubMed]
  4. T. Svensson, S. Andersson-Engels, M. Einarsdottır, and K. Svanberg, "In vivo optical characterization of human prostate tissue using near-infrared time-resolved spectroscopy," J. Biomed. Opt. 12, 014022 (2007). [CrossRef] [PubMed]
  5. A. Gibson, J. Hebden, and S. Arridge, "Recent advances in diffuse optical imaging," Phys. Med. Biol. 50, R1- R43 (2005). [CrossRef] [PubMed]
  6. C. Abrahamsson, A. Lowgren, B. Stromdahl, T. Svensson, S. Andersson-Engels, J. Johansson, and S. Folestad, "Scatter correction of transmission near-infrared spectra by photon migration data: Quantitative analysis of solids," Appl. Spectrosc. 59, 1381-1387 (2005). [CrossRef] [PubMed]
  7. F. Pandozzi and D. Burns, "Power law analysis estimates of analyte concentration and particle size in highly scattering granular samples from photon time-of-flight measurements," Anal. Chem. 79, 6792-6798 (2007). [CrossRef] [PubMed]
  8. T. Svensson, M. Andersson, L. Rippe, S. Svanberg, S. Andersson-Engels, J. Johansson, and S. Folestad, "VCSELbased oxygen spectroscopy for structural analysis of pharmaceutical solids," Appl. Phys. B 90, 345-354 (2008). [CrossRef]
  9. K. Yoo, F. Liu, and R. Alfano, "When does the diffusion-approximation fail to describe photon transport in random-media," Phys. Rev. Lett. 64, 2647-2650 (1990). [CrossRef] [PubMed]
  10. R. Haskell, L. Svaasand, T. Tsay, T. Feng, and M. Mcadams, "Boundary-conditions for the diffusion equation in radiative-transfer," J. Opt. Soc. Am. A 11, 2727-2741 (1994). [CrossRef]
  11. A. Hielscher, S. Jacques, L. Wang, and F. Tittel, "The influence of boundary-conditions on the accuracy of diffusion-theory in time-resolved reflectance spectroscopy of biological tissues," Phys. Med. Biol. 40, 1957-1975 (1995). [CrossRef] [PubMed]
  12. R. Cubeddu, A. Pifferi, P. Taroni, A. Torricelli, and G. Valentini, "Experimental test of theoretical models for time-resolved reflectance," Med. Phys. 23, 1625-1633 (1996). [CrossRef] [PubMed]
  13. A. Kienle and M. Patterson, "Improved solutions of the steady-state and the time-resolved diffusion equations for reflectance from a semi-infinite turbid medium," J. Opt. Soc. Am. A 14, 246-254 (1997). [CrossRef]
  14. E. Alerstam, S. Andersson-Engels, and T. Svensson, "White Monte Carlo for time-resolved photon migration," J. Biomed. Opt. (to be published). [PubMed]
  15. R. Graaff, M. Koelink, F. Demul, W. Zijlstra, A. Dassel, and J. Aarnoudse, "Condensed Monte Carlo simulations for the description of light transport," Appl. Opt. 32, 426-434 (1993). [CrossRef] [PubMed]
  16. A. Kienle and M. Patterson, "Determination of the optical properties of turbid media from a single Monte Carlo simulation," Phys. Med. Biol. 41, 2221-2227 (1996). [CrossRef] [PubMed]
  17. A. Pifferi, R. Berg, P. Taroni, and S. Andersson-Engels, "Fitting of Time-resolved reflectance curves with a Monte Carlo model," in Trends in Optics and Photonics: Advances in Optical Imaging and Photon Migration, vol. 2, pp. 311-314 (Optical Society of America, 1996).
  18. T. Svensson, E. Alerstam, M. Einarsdottır, K. Svanberg, and S. Andersson-Engels, "Towards accuracte in vivo spectroscopy of the human prostate," J. Biophoton. DOI: 10.1002/jbio.200710025 (posted 24 April 2008, in press).
  19. A. Pifferi, A. Torricelli, A. Bassi, P. Taroni, R. Cubeddu, H. Wabnitz, D. Grosenick, M. Moller, R. MacDonald, J. Swartling, T. Svensson, S. Andersson-Engels, R. van Veen, H. Sterenborg, J. Tualle, H. Nghiem, S. Avrillier, M. Whelan, and H. Stamm, "Performance assessment of photon migration instruments: the MEDPHOT protocol," Appl. Opt. 44, 2104-2114 (2005). [CrossRef] [PubMed]
  20. A. Pifferi, A. Torricelli, P. Taroni, D. Comelli, A. Bassi, and R. Cubeddu, "Fully automated time domain spectrometer for the absorption and scattering characterization of diffusive media," Rev. Sci. Instrum. 78, 053103 (2007). [CrossRef] [PubMed]
  21. B. Pogue and M. Patterson, "Review of tissue simulating phantoms for optical spectroscopy, imaging and dosimetry," J. Biomed. Opt. 11, 041102 (2006). [CrossRef] [PubMed]
  22. L. Spinelli, F. Martelli, A. Farina, A. Pifferi, A. Torricelli, R. Cubeddu, and G. Zaccanti, "Calibration of scattering and absorption properties of a liquid diffusive medium at NIR wavelengths. Time-resolved method," Opt. Express 15, 6589-6604 (2007). [CrossRef] [PubMed]
  23. M. Firbank, M. Oda, and D. Delpy, "An improved design for a stable and reproducible phantom material for use in near-infrared spectroscopy and imaging," Phys. Med. Biol. 40, 955-961 (1995). [CrossRef] [PubMed]
  24. J. Swartling, J. S. Dam, and S. Andersson-Engels, "Comparison of spatially and temporally resolved diffusereflectance measurement systems for determination of biomedical optical properties," Appl. Opt. 42, 4612-4620 (2003). [CrossRef] [PubMed]
  25. A. Pifferi, P. Taroni, G. Valentini, and S. Andersson-Engels, "Real-time method for fitting time-resolved reflectance and transmittance measurements with a Monte Carlo model," Appl. Opt. 37, 2774-2780 (1998). [CrossRef]
  26. R. Graaff, J. Aarnoudse, F. Demul, and H. Jentink, "Similarity relations for anisotropic scattering in absorbing media," Opt. Eng. 32, 244-252 (1993). [CrossRef]
  27. K. Furutsu and Y. Yamada, "Diffusion-approximation for a dissipative random medium and the applications," Phys. Rev. E 50, 3634-3640 (1994). [CrossRef]
  28. M. Bassani, F. Martelli, G. Zaccanti, and D. Contini, "Independence of the diffusion coefficient from absorption: Experimental and numerical evidence," Opt. Lett. 22, 853-855 (1997). [CrossRef] [PubMed]
  29. T. Nakai, G. Nishimura, K. Yamamoto, and M. Tamura, "Expression of optical diffusion coefficient in highabsorption turbid media," Phys. Med. Biol. 42, 2541-2549 (1997). [CrossRef]
  30. T. Durduran, A. Yodh, B. Chance, and D. Boas, "Does the photon-diffusion coefficient depend on absorption?" J. Opt. Soc. Am. A 14, 3358-3365 (1997). [CrossRef]
  31. L. Marti-Lopez, J. Hebden, and J.-L. Bouza-Dominguez, "Estimates of minimum pulse width and maximum modulation frequency for diffusion optical tomography," Opt. Laser Eng. 44, 1172-1184 (2006). [CrossRef]

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